a novel dissolution method for evaluation of polysaccharide based colon specific delivery systems a suitable alternative to animal sacrifice论文.pdfVIP

European Journal of Pharmaceutical Sciences 73 (2015) 72–80 Contents lists available at ScienceDirect European Journal of Pharmaceutical Sciences journal homepage: /locate/ejps A novel dissolution method for evaluation of polysaccharide based colon specific delivery systems: A suitable alternative to animal sacrifice Sachin Kumar Singh, Ankit Kumar Yadav, G. Prudhviraj, Monica Gulati ⇑, Puneet Kaur, Yogyata Vaidya School of Pharmaceutical Sciences, Lovely Professional University, Phagwara 144411, Punjab, India a r t i c l e i n f o a b s t r a c t Article history: The most extensively used test for predicting in-vivo release kinetics of a drug from its orally adminis- Received 31 January 2015 tered dosage forms is dissolution testing. For polysaccharide based, colon targeted oral delivery systems, Received in revised form 20 March 2015 the entire path of the gut traversed by the dosage form needs to be simulated for assessing its in-vivo Accepted 20 March 2015 dissolution pattern. This includes the dissolution testing sequentially in simulated gastric fluid (SGF), Available online 28 March 2015 simulated intestinal fluid (SIF) and simulated colonic fluid (SCF). For SGF and SIF, simple and standardized composition is well-known. However, preparation of SCF requires addition of either the colonic contents Keywords: of rodents or human faecal slurry. A method is proposed, wherein a mixture of five probiotics cultured