生物法同步脱硫除氮的研究 毕业论文.doc

目录 中文摘要1 英文摘要………………………………………………………………………………2 1 引言…………………………………………………………………………………3 1.1 脱氮硫杆菌的基本生长条件和脱硫除氮机理………………………………4 1.2 脱氮硫杆菌的筛选和性能研究………………………………………………4 1.3 脱氮硫杆菌的应用……………………………………………………………5 1.4 尚待研究的问题………………………………………………………………8 1.5 研究的意义……………………………………………………………………8 2 材料与方法………………………………………………………………………10 2.1 培养基的组成………………………………………………………………10 2.2 土样来源……………………………………………………………………10 2.3 试剂…………………………………………………………………………10 2.4 主要仪器……………………………………………………………………11 2.5 方法…………………………………………………………………………11 2.6 指标检测方法………………………………………………………………13 3 结果与分析………………………………………………………………………15 3.1 硫酸根标准曲线……………………………………………………………15 3.2 硝酸盐氮校准曲线…………………………………………………………15 3.3 同一硝酸盐浓度下不同硫氮比的同步脱硫除氮效果……………………16 3.4 最佳硫氮比下不同硝酸盐浓度的同步脱硫除氮效果……………………22 结论与展望…………………………………………………………………………27 谢辞…………………………………………………………………………………29 参考文献……………………………………………………………………………30 生物法同步脱硫除氮的研究 摘在厌氧条件下从土壤中富集出脱氮硫杆菌混菌，脱氮硫杆菌Na2S2O3·5H2O、Na2S·9H2O作为还原态硫来源，在相同接种量、不同硫氮比下，考察其硝酸盐氮浓度降到10mg/L的时间长短和硝酸盐氮去除率高低，时间短、去除率高则为同步脱硫除氮的最佳硫氮比。其次是，在黄铁矿、Na2S2O3·5H2O、Na2S·9H2O的最佳硫氮比下，使用相同的接种量，分别在不同的硝酸盐浓度下，考察其硝酸盐氮去除率高低，去除率高则为同步脱硫除氮的最佳硝酸盐浓度。试验结果表明，黄铁矿、Na2S2O3·5H2O、Na2S·9H2O的最佳硫氮比分别为33和2.5最佳硝酸盐浓度分别为45、1525mg/L。关键词：脱氮硫杆菌；硫氮比；反硝化；生物法；同步法 Simultaneous biological nutrient removal study of desulfurization Abstract: Thiobacillus denitrification under anaerobic conditions in the desulfurization with simultaneous physiological characteristics in addition to nitrogen, under anaerobic conditions in soil enrichment from mixed flora were selected in the S-N ratio under different pyrite, Na2S2O3·5H2O, Na2S·9H2O to restore the state of sulfur sources, the best study of its S-N ratio. N than in the best of different concentrations of nitrate pyrite, Na2S2O3·5H2O, Na2S·9H2O desulfurization in addition to the effects of nitrogen. The results showed that the concentration of nitrate under certain conditions, pyrite, Na2S2O3·5H2O, Na2S·9H2O best 3,3,2.5 N ratio, respectively. N than in the best conditions, pyrite, Na2S2O3·5H2O, Na2S·9H2O best nitrate concen