水飞蓟宾A的水飞蓟宾B在大鼠体内的药动学及生物利用度研究.pdfVIP

水飞蓟宾A 与水飞蓟宾B在大鼠体内的药动学及生物利用度 研究 * 褚扬，李伟，万梅绪，栗志文，郭嘉华，马晓慧，周水平 (天津天士力集团研究院药理毒理所，天津 300410) [摘要] 目的：建立一种简单、快速、灵敏的液-质联用法(LC-MS/MS)，同时 测定大鼠血浆中水飞蓟宾A 与水飞蓟宾B 浓度。方法：0.1 mL 血浆样品经沉淀 蛋白预处理后，以甲醇-0.1%甲酸(48:52，v/v)为流动相，采用Zorbax Eclipse XDB-C18 色谱柱(150 × 2.1 mm，5 μm)分离，以ESI 源负离子模式、选择反应 监测(SRM)方式进行定量分析。结果：水飞蓟宾A 与水飞蓟宾B 在血浆中的线性 -1 -1 范围为2.0~50000 ng ·mL ，最低定量限(LLOQ)为2.0 ng ·mL 。药物的日内、日 间精密度在 9.1%以内，准确度(相对误差)小于±5.7%。结论：本方法成功地用 于水飞蓟宾A 与水飞蓟宾B 各自的药动学及生物利用度研究。 [关键词]水飞蓟宾A；水飞蓟宾B；液-质联用；药动学；生物利用度 [中国图书分类号]R 969 [文献标识码]A Investigation of the bioavailability and pharmacokinetics of silibinin A and silibinin B in rats CHU Yang, LI Wei, WAN Mei-xu, LI Zhi-wen, GUO Jia-hua, MA Xiao-hui, ZHOU Shui-ping (Tasly RD Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China) [ABSTRACT] OBJECTIVE： To develop a simple, rapid and sensitive 作者简介: 褚扬(1982-) ，男，硕士 研究方向: 药物代谢及药动学 Tel.: (022) Fax: (022) E-mail: chuyang0610@163.com LC/MS/MS method for the determination of silibinin A and silibinin B in rat plasma. METHODS ：Sample pretreatment involved in one-step protein precipitation with acetonitrile of 0.1 mL plasma. The separation of silibinin A and silibinin B was performed on a Zorbax Eclipse XDB-C column 18 (150 × 2.1 mm, 5 μm) with the mobile phase of methanol-water-formic acid (48:52:0.1, v/v/v). The analytes were monitored with negative electrospray ionization (ESI) by selected reaction monitoring (SRM) mode. RESULTS ：The linearity of this method was found to be from 2.0 to 50000 -1