启膈方对人胃癌MGC细胞增殖、运动能力及分泌MMP-2、MMP-9酶活性的影响_临床医学论文.docVIP

启膈方对人胃癌MGC细胞增殖、运动能力及分泌MMP-2、MMP-9酶活性的影响_临床医学论文 启膈方对人胃癌MGC细胞增殖、运动能力及分泌MMP-2、MMP-9酶活性的影响_临床医学论文 作者：李晶,李娜,刘亚娴,卢付河,付晓梅 【摘要】 目的 观察启膈方对人胃癌MGC细胞增殖能力、运动能力及分泌基质金属蛋白酶(MMP)-2、MMP-9活性的抑制作用。方法 体外培养人胃癌MGC细胞,随机分为启膈方小、中、大剂量组及5-氟尿嘧啶(5-FU)组、空白对照组,共5组。启膈方小、中、大剂量组加入不同浓度的中药,其终浓度为35、70、140 mg/mL;5-FU组加入5-FU使其终浓度为50 ?g/mL,空白对照组加入PBS。分别用MTT法检测肿瘤细胞的增殖,琼脂滴法检测肿瘤细胞运动能力,明胶酶谱法检测肿瘤细胞分泌MMP-2、MMP-9的活性。结果 启膈方对MGC细胞体外增殖有明显的抑制作用,并随着药物浓度增加其抑制作用更加明显。启膈方各剂量组、5-Fu组运动能力较对照组明显弱,运动速度慢,差异有统计学意义(0.05)。启膈方各剂量组细胞分泌MMP-2、MMP-9活性明显较空白对照组减弱(0.05),其中MMP-2活性减弱更明显(0.01)。结论 启膈方可通过抑制MGC肿瘤细胞的增殖、运动能力及MMP-2、MMP-9的活性来抑制转移。 【关键词】 启膈方;细胞培养;细胞增殖;细胞运动;基质金属蛋白酶-2;基质金属蛋白酶-9 　　Abstract：Objective To observe the inhibitory action of Qigefang on cell proliferation, movement, secreting MMP-2 and MMP-9 of MGC gastric cancer cell line. Methods MGC cell line of gastric cancer was cultured and divided into five groups randomly：low-, middle-, high-dose group of Qigefang, 5-FU group, control group. Qigefang was added in Qigefang group to make final concentration of 35, 70, 140 mg/mL, 5-FU in chemothapy group to 50 ?g/mL, and PBS in control group. Tumor cell proliferation was detected by MTT, moving ablity of tumor cell by agar drop, and MMP-2, MMP-9 activity by gelatinase zymography. Results Qigefang had obviously depressant effect on proliferation of MGC cell. It became more obviously with raising drug density. Moving ability of all dose Qigefang groups and 5-FU group were more feeble than control group. The difference was significant (0.05). Activity of MMP-2 and MMP-9 in all dose Qigefang group was weaker than control group (0.05), especially the activity of MMP-2 (0.01). Conclusion Qigefang can inhibit metastasis of MGC cell by inhibiting its function of proliferation, movement, secreting MMP-2 and MMP-9. 　　Key words：Qigefang;cell culture;cell proliferation;cell movement;MMP-2;MMP-9 　　胃癌发生转移是一个极其复杂的多基因调控和多步骤发生过程,涉及癌细胞增殖、细胞粘附、基质降解、细胞迁移等许多环节[1]。胃癌就其临床表现可归属于中医的“噎膈”、“反胃”等范畴,本研究将《医学心悟》中治疗噎嗝的启膈散化裁而成启膈方,体外作用于胃癌MGC细胞,从细胞增殖、运动、分泌降解酶3个方面,来探讨启膈方抗胃