复合抗氧化剂对荷瘤大鼠60 Coγ射线局部照射时血液氧化损伤的影响_临床医学论文.docVIP

复合抗氧化剂对荷瘤大鼠60 Coγ射线局部照射时血液氧化损伤的影响_临床医学论文 复合抗氧化剂对荷瘤大鼠60 Coγ射线局部照射时血液氧化损伤的影响_临床医学论文 作者：秦绪军　何伟　海春旭　梁欣　张晓迪　赵康涛　陈宏莉　刘瑞 【关键词】 荷瘤鼠, 关键词: 抗氧化剂;60 Coγ射线;照射;氧化损伤;荷瘤鼠 摘 要:目的 探讨荷瘤大鼠60 Coγ射线局部照射时血液学氧化损伤以及复合抗氧化剂抗损伤作用. 方法 采用Walk-er-256肿瘤细胞株接种大鼠皮下，得到实体瘤，摘除实体瘤分割成小块植入大鼠的右后腿皮下，制成大鼠荷瘤模型，将荷瘤大鼠分成3组，分别为肿瘤组、照射组和抗氧化剂保护组，同时选取正常大鼠作为阴性对照组.抗氧化剂保护组每日给予复合抗氧化剂ig，分次对照射组和抗氧化剂保护组的荷瘤大鼠进行60 Coγ射线局部照射4次，每次间隔1wk，累计剂量为47Gy，最后一次照射后7d，活杀大鼠，取全血和血清测定过氧化氢酶（CAT）、丙二醛（MDA）、总超氧化物歧化酶（T-SOD）和总蛋白. 结果 照射组的MDA含量显著高于非照射组和抗氧化剂保护组（347μmol g-1 vs292，305和196μmol g-1 ，0.05），复合抗氧化剂显示出良好的保护作用.而照射组的抗氧化酶类如总超氧化物歧化酶（T-SOD）活性显著低于非照射组和抗氧化剂保护组（3.65μkat g-1 vs4.05，4.82和4.70μkat g-1 ，0.05），CAT的活性在照射组显著低于肿瘤组和抗氧化剂保护组（362K g-1 vs677和672K g-1 ，0.05）. 结论 60 Coγ射线局部照射可以产生自由基引发的全身氧化损伤，高效的复合抗氧化剂可以起到有效的保护作用. Keywords:antioxidants;60 Coγ-irradiation;radiation;oxida-tive injury;rats with tumor 　　Abstract:AIM To observe the oxidative injury of rats with tumor by local60 Coγ-irradiation and the protection of antiox-idants compound.METHODS The SD rats were divided in-to control group as negative control group（NCG）and experi-mental groups（TG，RG and PG）.The tumor cell strain Walker-256was injected into the rat to get the entity tumors which were cut into small pieces and were embedded into the right rear buttocks under skin of rats.As the embedded tu-mor had grown up successfully，they were randomly divided into3groups which were tumor group（TG），radiation group（RG）and radiation plus protection group（PG）.The PG was given antioxidants compound by gavage and other groups were given the same dosage water.RG and PG were locallyγ-irradiated with the total doses of47Gy，then all the rats were sacrificed and the bloods collected to measure the con-tent of serum malondialdehyde（MDA），the activities of the total superoxide dismutase（T-SOD）and the catalase（CAT）.RESULTS The content of MDA in RG is much higher than that in all the other groups（347μmol g-1 vs292，305and196μmol g-1 ，0.05）.The activities of SOD and CAT in RG are much lower than those in the T