复方中药复明片对兔视网膜脱离后视网膜组织形态的影响_临床医学论文.docVIP

复方中药复明片对兔视网膜脱离后视网膜组织形态的影响_临床医学论文 复方中药复明片对兔视网膜脱离后视网膜组织形态的影响_临床医学论文 作者：彭清华，刘娉，彭俊，姚小磊，谭涵宇 【摘要】 　　目的：研究复方中药复明片对兔实验性视网膜脱离后视网膜组织形态结构的影响。方法：用有色家兔72只，设正常对照A组、模型B组、西药对照C组、复明片D组，每组9只。B，C，D组制作视网膜脱离模型。造模后第1d下午开始给药，A组与B组均予温开水灌胃，C组予西药混合溶液灌胃，D组予复明片混悬液灌胃，皆5mL/kg，1次/d。造模后7，14，21d观察各组视网膜复位情况，取材后光镜下观察视网膜组织形态学结构变化。结果：复明片能促进脱离视网膜复位,减轻视网膜组织及细胞的形态学损伤，改善视功能。结论：复明片有保护视网膜组织结构的作用。 【关键词】 复明片；益气养阴活血利水法；视网膜脱离模型；组织结构；兔 　　Astract　AIM: To investigate effects of fuming tablets(FMT) on morphology of retinal tissue after retinal detachment(RD) of rabbits. METHODS: Seventytwo rabbits were divided into normal group (group A), model group (group B), western medicine group(group C), FMT group(group D). Each group had 9 rabbits.Group B,C,D were made RD model. After models were made one day they were given medicine. Group A and B were administrated intragastrically with warm water. Group C was administrated intragastrically with western medicine solution. Group D was administrated intragastrically with FMT suspension, once a day. After models were made, the retina recovery and the changes of morphology of retinal tissue on the 7th, 14th, and 21st day, were observed with microscope. RESULTS: FMT can promote the retinal reattachment, reduce the morphology damage of retina cells and tissue and improve sight. CONCLUSIONS: FMT has effects of protecting retinal tissue structure 　　KEYWORDS: fuming tablets; method of qibenefitting, yinfostering, and blood invigorating and diuresisactivating; retinal detachment model; tissue structure; rabbit 　　0　引言 　　益气养阴活血利水法能促进视网膜脱离(retinal detachment, RD)术后患者视网膜的复位，提高其术后视功能[1]。为了探讨其作用机制，我们观察具有益气养阴活血利水作用的复明片对兔实验性视网膜脱离后视网膜组织形态结构的影响如下。 　　1　材料和方法 　　1.1　材料 　　成年健康有色家兔72只，雌雄各半，由湖南中医药大学实验动物中心提供。复明片由黄芪、生地、茯苓、车前仁、地龙等益气养阴活血利水中药按现代制剂制备工艺制成，由湖南中医药大学第一附属医院药剂科提供。使用时研成粉末，温开水混合成浓度为100g/L的混悬液。温开水混合成每升水含VitB 1276mg，ATP 1102mg，维脑路通734mg的溶液。 　　1.2　方法 　　造模[2]后1d下午开始给药，A正常对照组与B模型对照组均予温开水灌胃，C西药对照组予西药混合溶液灌胃，D复明片治疗组予复明片混悬液灌胃，皆5mL/kg，1次/d。造模后7，14，21d，各小组取对应兔6眼，用30g/L戊巴比妥1mL/kg耳缘静脉注射麻醉兔，摘除