发酵和酶解共处理玉米秸秆研究.pdfVIP

ofAnhui 安徽农业科学，Journal Agri．Sci．2010，38(19)：10484—10485。10488责任编辑熊章琴责任校对李岩 发酵和酶解共处理玉米秸秆研究 曲源，王修俊+，剥、倩 (贵州大学化学与化T学院，贵州贵阳550003) 摘耍[目的]探究发酵和酶解共处理玉米秸秆效果。[方法]利用有效微生物发酵玉米秸秆，并对发酵后玉米秸秆进行酶解。采用单 因素法，考察温度、pH值、酶与底物比、处理时间对酶解效果的影响。[结果]发酵和酶解单独处理玉米秸秆，效果均不理想；有效微生物 群可以软化秸秆，利于酶解。在温度为50℃、pH值为4．8、酶与底物比为15g／kg、处理时间为72h的条件下，纤维素酶酶解发酵后的秸 秆所得总糖有较大幅度提高，还原糖提高较小。，[结论]该研究为玉米秸秆综合利用及饲料开发提供了试验依据。 关键词有效微生物群；发酵；酶解；玉米秸秆 中图分类号S216．2文献标识码A 文章编号0517—6611(20lo)19—10484—02 ontheTreatmentofCOrBStalkwiththeMethodofFermentationand PreliminaryStudy Enzymolysis Yuanet of andChemical Chemistry 550003) QU al(College Engineering，GuizhouUniversity，Guiyang，Guizhou Abstract researchonthetreatmentofcornstalkwiththemethodoffermentation studied and [Objective]The enzymolysisw船preliminarily inthe Method1 111ecoinstalkwasfermentedeffective and afteritsfermentation．Thesingle．factor experiment．f by microorganismhydrolyzed wereusedto theinfluenceof of resultsindicated experiment study temperature。pH，ratioenzyme—substrate，treatmenttime．『Result】The thattheeffectoftheindividualtreatmentoffermentationor oncoinstalkwas effective couldsof- enzymolysis unsatisfactory．Themicroorganism tenthestalk．whichbe forits increasedandthe w觞increasedinthe would total was helpful enzymolysis．Thesugargreatly reducingsugar C0131stalkafter underthe conditions：the ratioof and enzymolysisfollowing temperature，30℃；t}1epH，4．8；the enzyme—substrate，159／kg thetreatmenttime，72hours．fConclusion】ne evidenceforthe utilizationofcornstalkandthe of