醋酸棉酚抑制人骨肉瘤MG-63 细胞增殖.pdfVIP

162 食品与药品 Food and Drug 2011年第 13卷第05期 醋酸棉酚抑制人骨肉瘤MG-63细胞增殖 徐万龙，孙治国，王 浩，袁 宏* （新疆维吾尔 自治区人民医院骨外一科，新疆 乌鲁木齐 830000 ） 摘 要：目的 研究醋酸棉酚 （gossypol acetic acid ，GAA ）对人骨肉瘤MG-63细胞增殖的影响及其可能作用机制。方法 采用MTT法检测GAA 对MG-63细胞的增殖抑制作用，Hoechst 33258荧光染色观察细胞核的形态变化，流式细胞仪分析 细胞凋亡相关蛋白Bcl-2与Bax 的表达情况。结果 GAA对MG-63细胞具有显著的增殖抑制作用，并呈剂量、时间依赖性； Hoechst 33258荧光染色可观察到明显核固缩、染色质凝集等细胞凋亡现象；流式细胞仪检测发现细胞凋亡相关基因Bcl-2 表达下调，而Bax表达则相对上调。结论 GAA 能够抑制人骨肉瘤MG-63细胞的增殖，促进其凋亡，其作用机制可能是通 过调节凋亡相关蛋白Bcl-2 、Bax实现。 关键词：醋酸棉酚；人骨肉瘤MG-63细胞；细胞增殖 中图分类号：R969 文献标识码：A 文章编号：1672-979X （2011）05-0162-03 Mechanism of the Inhibitory Action of Gossypol Acetic Acid on the Proliferation of Osteosarcoma MG-63 Cells in Vitro XU Wan-long, Sun Zhi-guo, Wang Hao, Yuan Hong (Dep artment of Bone Surgery 1, Peop le ’s Hosp ital of X inj iang Uygur Autonomous Region, Urumqi 830011,China ) Abstract: Objective To investigate the mechanism of the inhibitory action of gossypol acetic acid(GAA) on the proliferation of osteosarcoma MG-63 cells in vitro. Methods The inhibition of proliferation of GAA in MG-63 cells was examined by MTT analyzes. The morphology changes of the nucleus were observed by Hoechst 33258 staining. The expression of apoptosis-associated gene protein was detected by flow cytometer. Results The proliferation of MG- 63 cells was significantly inhibited by GAA in a dose-dependent and time-dependent manner. Distinct morphologic changes of cell apoptosis such as karyopyknosis and chromatic agglutination were observed by Hoechst 33258 staining. The expression of Bcl-2 was down-regulated while Bax was up-regulated. Conclusion GAA could inhibit proliferation of the human osteosarcoma MG-63 cells and induce apoptosis, with the mechanism of which could be related to the regulation of Bcl-2 and Bax. Key Words: gossypol acetic