胃癌组织中nm-23-H1、c-Met和survivin蛋白表达及临床意义.pdfVIP

·大会交流· 胃癌组织中nm-23-H1、c-Met 和survivin 蛋白表达及临床意义 鲁凯 姚壮凯 刘燕文 赵成 刘永平 基金项目：连云港卫生局科研项目（07025 ） 222002 连云港，江苏连云港第二人民医院普外科 【摘要】 目的 探讨胃癌组织中nm23-H1 、c-Met、survivin蛋白表达及其临床意义。方 法 对50例胃癌蜡快标本，运用S-P免疫组化技术检测nm23-H1 、c-Met、survivin蛋白表达情 况。并分析各基因表达与胃癌组织的分化程度、浸润深度、淋巴结转移、TNM分期及转移和 复发之间的关系。结果 nm23-H1 、c-Met、survivin蛋白表达率分别为46% 、72%、80%，胃 癌TNM分期、淋巴结转移与nm23-H1蛋白低表达有密切关系，c-Met蛋白的高表达与胃癌TNM 分期、淋巴结转移有密切关系，survivin蛋白的高表达与胃癌分化程度、TNM分期、淋巴结转 移有密切关系，nm23-H1蛋白表达与survivin表达呈负相关，c-Met蛋白的表达与survivin表达呈 正相关。结论 nm23-H1蛋白低表达和c-Met、survivin高表达对于早期诊断和预后分析有重要 指导意义，据其表达开展有选择性地基因治疗应有一个良好的应用前景。 【关键词】 胃癌；nm23-H1 ；c-Met ；survivin；淋巴结转移；免疫组织化学 【中图法分类号】 R735.2 【文献标识码】 B Expression and Clinical Significance of nm23, c-Met and survinvin protein in Gastric Carcinoma Tissue LU Kai, YAO Zhuang-kai, LIU Yan-wen, ZHAO Cheng, LIU Yong-ping. Department of General Surgery, the Second People’s Hospital of Lianyungang, Lianyungang 222023, China 【Abstract 】 Objective To Study the expression and clinical significance of nm23-H1, c-Met and survinvin protein in gastric carcinoma tissue. Method Expression of nm23-H1, c-Met and survinvin protein in 50 paraffin block samples of gastric carcinoma was measured by S-P immunohistochemistry staining. The relationship between each expression and tumor differentiation degree, infiltration depth, lymph node metastasis, TNM stage, tumor metastasis and recurrance were analyzed. Results The positive expression rate of nm23-H1, c-Met and survinvin protein was 46%, 72% and 80%, low nm23-H1 protein expression and high c-Met protein expression correlated closely with TNM stage and lymph node metastasis of gastric carcinoma, high survinvin protein expression correlated closely with differentiation degree, TNM sta