3T3-L1前脂肪细胞与蚕丝支架的体外共培养.pdfVIP

中国组织T程研究与临床康复彳争J2眷彳萝Ⅳ嬲2008—04—0l出版 Journal 1issue ofClinicalRehabilitative J。2008I／01．12,No．14 EngineeringResearch却ril 3T3．L1前脂肪细胞与蚕丝支架的体外共培养★ 刘秀华 Culture of3T3-LI onsilkscaffolds／nv／fro preadipocytes UUXiu．hua Abstract：Silkhas mechanical and to materials 警叩1of掣?崛 good performancesuperiorbiocompatihilitydegradablepolymer observestheeffectsofsilkonthe andfunctionof3T3一LI naturalsilkand 竺“翌 紫守 synthetic．协sstudy attachment，shape preadipocytes．The silk were into of tlle three．dimensionalsc舶ldS．3T3．Ll atthe 梨舞竺蒿： digestedbyqpsincompounded preadipocytecellSuspensiondensity 6×10’oL‘wasalso cell wereinoculatedonthesilkscaffoldsandculturedfor卜4weeks．Itwas Province．China。‘ prepared；3T3一LIsuspensions observedunderinverted that3T3一LI extendedslim and forwardthe ．．… ．…． microscopy preadipocytes processesmigrated alongsilk，ceils head．tailinteractionandthenfuseato intheholesofthesilk．Thecellsattachedthescaffolds 攀砷一hu璺’c唑presented piecesgradually tightly， ；xtended andsecretedextracellularmamx，whichwereobserved electron ：：娑”．Sc船墨 appropriately byscanning microscopy．Silkcompound for function scaffoldisideal and maintenanceof3T3·LI inthree-dimensionalculture． Medicine．Huzlmu attachment,morphology preadipocytes Teach