内稳态人胚胎干细胞向成熟功能细胞诱导分化特征的研究.pdfVIP

vitroandtheirdevelopmentwasevaluated.Results Atotalof194blastomereswereisolatedfrom52 normallyfertilizedhumanlow-qualityembryos,thecleavagerateofblastomereswas57.7％,41.2％ of theblastomerescoulddevelopto3～4cellstage,29.4％ oftheblastomerescoulddeveloptocompaction stage,4.1％ oftheblastomerescoulddeveloptotrophectoderm-likeoutgrowth.Thereweretwohuman embryonicstemcelllinesderivedattherateof1.0％ andbothofthemmaintainednormalkaryotype, expressedthetypicalstemnessmarkersandshowntotipotentphenotypeincludingtheabilitytodifferen tiateintoallthreegermlayersinvitroandinvivo.Conclusion Thisi