DGGE技术分析接种菌剂在猪粪堆肥中动态变化.pdfVIP

中国畜牧兽医学会动物微生态学分会第四届第九次学术研讨会论文集(1-@r)(2008年河南新乡) DGGE技术分析接种菌剂在猪粪堆肥中的动态变化 王芳7·1一，倪学勤2s，曾东2白林3马志勇2 (1．四川农业大学资源与环境学院，四川雅安625014；2．四川农业大学动物医学院动物微生态研 究中心，四川雅安625014) 摘要：通过PCR-DCJGE技术研究接种茵剂和自然发酵的猪粪堆肥中细菌群落的动态变化．结果表明。自 然发酵的猪粪堆肥中土著细菌的种类较丰富，到堆肥结束时，与接种菌剂的堆肥相比其细菌群落组成变化 不大；而在堆肥中接种菌剂后，堆肥第2天就成为高温期中的优势茵群，提高了堆肥的温度，延长了高温 持续期，并且使原有的微生物群落结构发生较大的改变。研究表明堆肥中添加茵剂是提高堆肥温度和加快 堆肥腐熟的有效方法，应用PCR-DGGE技术可以很直观准确地研究添加菌剂在堆肥中的动态变化． 关键词：堆肥；复合菌剂；DGGE：微生物群落 of GradientGel on ofMicrobial ApplicationDenaturing ElectrophoresisStudy Inoculated of manure during Compostspig Dynamics Don92，Ma WangFang啦,NiXue．qin2+，Zeng Zhi．yon92 ofResourcesandEnvironmentalSciences，SichuanAgricultureUniversity,Ca’柚，Sichuan625014，aIiIl矗； (1college ofAnimalScience，SichuanAgricultureUniversity,Ya’an,Sichuan 2college 625014，China) ofmicrobial inoculatedandnatural were Abstract：Microbial community dynamics during composts withPCR andthemethodsof gradient investigatedtechnology denaturinggel a resultsindicatedthatnatural had of microbial varietymicroorganisms；the composts community, withinoculated li砌euntilthetest inoculatedstrains compared compostchanged completed．However．the assumed the after themicrobial predominanceduringhightemperaturephase inoculating，and community had alot．Theresultshowsthat isalleffectivetoraise andshortenthe