海南臭黄荆醇提物的抗氧化活性作用.pdfVIP

第29卷 第2期 医 学 研 究 与 教 育 Vol.29 No.2 2 0 1 2 年 4 月 Medical Research and Education Apr.2012 海南臭黄荆醇提物的抗氧化活性作用 1,2 2 1,2 戴春燕 ，周君，莫峥嵘 （1. 教育部热带药用植物化学省部共建重点实验室，海南 海口 571158；2. 海南师范大学化学与化工学院，海南 海口 571158） 摘要：目的 研究海南臭黄荆根、茎、叶各部位总提物的抗氧化活性。 方法 根据DPPH·体系吸光值的变化研究海南 臭黄荆清除自由基的能力，DPPH·反应体系为5 mL 6.5×10-5 mol/L DPPH· + 1 mL样品溶液，反应时间30 min。考 察了海南臭黄荆不同浓度根、茎、叶醇提物与DPPH·自由基清除率的关系。 结果 对DPPH·自由基的清除能力： IC50(根)=2.315×10-3 g/mL，IC50(茎)=2.440×10-3 g/mL，IC50(叶)=1.004×10-3 g/mL。 结论 海南臭黄荆醇提物具有抗氧化活 性，可以进一步开发为天然抗氧化剂。 关键词：海南臭黄荆；自由基清除率；抗氧化活性；DPPH 中图分类号：R282.71 文献标志码：A 文章编号：1674-490X(2012)02-0078-04 Antioxidant activity of alcohol extraction from Premna hainanensia 1, 2 2 1, 2 DAI Chunyan , ZHOU Jun , MO Zhengrong (1. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Haikou 571158, China; 2. College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China) Abstract: Objective To study the antioxidant potential of alcohol extraction from the root, stem and leaf of Premna hainanensia by studying absorption spectrum of DPPH• reaction system. Methods The condition was drawn as follows, DPPH• reaction system was 5 ml of 6.5 ×10-5 mol/L DPPH• + 1 ml of sample solution with the reaction time of 30 min. The relation between DPPH• radical scavenging rate and the concentration of alcohol extraction were studied, and IC50 (the value of antioxidant concentration for scavenging half of DPPH• radical) was used as the index to evaluate scavenging capacity. Results The determining IC50 values were as follows: IC50 -3 -3