呼吸机相关肺炎与气管内导管生物被膜相关性研究.pdfVIP

实用医药杂志 年 月第 卷第 期 · · 2010 12 27 12 Prac J Med ＆ Pharm． Vol 27, 2010－12 No．12 1057 临床研究 ·论 著· 呼吸机相关肺炎与气管内导管生物被膜相关性研究 王 瑛，张劭夫 摘 要 目的 探讨呼吸机相关肺炎（ ）致病菌与气管内导管生物被膜的形成状况。 方法 通过结晶紫染 [ ] VAP 色鉴定 例 患者气管导管生物被膜形成情况并分析与分泌物培养致病菌分布的相关性。 结果 革兰阴性杆 15 VAP 菌为 的主要致病菌， 占 ， 例 气管导管形成生物被膜 例，其中以铜绿假单胞菌、鲍曼不动杆菌和 VAP 66% 15 VAP 7 流感嗜血杆菌为主。 结论 机械通气相关肺炎细菌分布与气管插管形成生物被膜有一定相关性。 关键词 呼吸机相关肺炎；气管内导管；生物被膜 [ ] [ 中图分类号] R563.1 [文献标识码] A Correlation between ventilator associated pneumonia and endotracheal tube biofilm inoculation WANG Ying, ZHANG Shao-fu. Dept. of Respiratory disease the General Hospital of Jinan Militay Region, Jinan, Shandong 250031, China [Abstract] Objective To observe the formation of biofilms inside tracheal tubes and to evaluate the correlation between the distribution of pathogens in patients with ventilator-associated pneumonia(VAP). Methods The secretion of lower respiratory tract of 15 patients was cultured with a quantitative method. Formation of bacterial biofilm inside tracheal tubes was examined by crystal violet assay. Results Gram-negative bacilli (GNB) （66% ） were the main pathogens in VAP. Of 15 endotracheal tubes, in 7 tubes there was observed biofilm formation, in which Pseudomonas aeruginosa, Acinetobacter baumannii and Haemophilus were the main pathogens. Conclusion There is a close connection between the formation of bacterial BF and the distribution of pathogens in patients with ventilator-associated pneumonia (VAP). [Key words] Ventilator associated pneumonia(VAP);