黄花蒿提取物对柑橘全爪螨的生物活性.pdfVIP

中国农业科学 2009,42(6):2217-2222 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.06.044 黄花蒿提取物对柑橘全爪螨的生物活性 张永强，丁 伟，田 丽，赵志模 （西南大学植物保护学院，重庆 400716 ） 摘要：【目的】通过系统测定黄花蒿对柑橘全爪螨的杀螨生物活性，为综合开发利用黄花蒿提供科学依据。【方 法】采集 6 月份和 7 月份的黄花蒿植株，分成根、茎、叶 3 个部分，采用石油醚（30～60℃）、石油醚（60～90 ℃）、乙醇、丙酮和水溶剂的平行和顺序提取方法，获得 54 种提取物，并测定了它们对柑橘全爪螨的室内杀螨活 性。【结果】结果表明，7月份的黄花蒿植株的生物活性大多优于6月份，并以7月份叶的丙酮平行提取物活性最 高。对柑橘全爪螨处理48 h后，7月份叶的丙酮平行提取物对柑橘全爪螨的LC -1 50（0.4222 mg·ml ）仅为6月份（0.9489 mg·ml-1 ）的44%。对黄花蒿7 月叶的丙酮提取物进行柱层析得20种不同的组分，其中组分17的杀螨活性最高，与 其它组分的杀螨活性存在显著差异（P ＜0.05）。【结论】7月叶丙酮平行提取物对柑橘全爪螨的生物活性最强。 并对其进行柱层析分离，得20种组分，组分17的杀螨活性最高，具体的杀螨活性化合物还有待进一步深入研究。 关键词：黄花蒿；柑橘全爪螨；杀螨生物活性 Acaricidal Bioactivity of Artemisia annua Extracts Against Panonychus citri (Acari: Tetranychidae) ZHANG Yong-qiang, DING Wei, TIAN Li, ZHAO Zhi-mo (College of Plant Protection, Southwest University, Chongqing 400716) Abstract: 【Objective 】 To determine the acaricidal bioactivities of Artemisia annua against Panonychus citri,so to provide a scientific basis for the comprehensive development and utilization of A. annua . 【Method 】 Collect The whole A. annua plants were collected in June and July respectively, and the whole plants were cut into root, stems and leaves, petroleum ether (30-60 ℃), petroleum ether (60-90 ℃), ethanol, acetone and water were used and extracted by the parallel and sequenced solvents extraction methods, finally 54 kinds of extracts were obtained and their indoor acaricidal bioactivity against P. citri were determined. 【Results 】 The results showed