基于柑橘及其近缘属植物DNA 条形码的叶绿体编码序列筛选.pdfVIP

中国农业科学 2011,44(2): Scientia Agricultura Sinica 基于柑橘及其近缘属植物DNA条形码的叶绿体编码序列筛选 1 1 3 1，2 于 杰 ，闫化学 ，鲁振华 ，周志钦 1 2 3 （西南大学南方山地园艺学教育部重点实验室, 重庆 400715 ；西南大学园艺园林学院，重庆 400716 ；中国农业科学院郑州果树研究所，郑州 450009 ） 摘要：【目的】通过对柑橘及其近缘属植物叶绿体 4 种编码序列的测定分析，获得能进行 DNA 条形编码的特 征序列，为进一步研究叶绿体非编码区序列奠定基础。【方法】对柑橘及其近缘属植物59份样品进行matK、rpoB、 rpoC1、rbcL 测序，序列比对与人工校正，计算属间、种间、种内的遗传距离，比较序列间的差异，建立系统发 育树。【结果】4种序列中， matK序列在属间、种间差异最大，与其它序列相比具有显著性差异，rbcL序列次之， 而 rpoB、rpoC1 序列两者间没有显著性差异。【结论】matK 序列是柑橘及其近缘属植物 DNA 条形码的未来研究中 一个重要的候选片段。 关键词：柑橘属；近缘属；DNA条形码；编码序列 Screening Potential DNA Barcode Regions of Chloroplast Coding Genome for Citrus and Its Related Genera 1 1 3 1,2 YU Jie , YAN Hua-xue , LU Zhen-hua , ZHOU Zhi-qin 1 ( Key Laboratory of Horticulture Science for Southern Mountainous Regions ，Ministry of Education, Southwest University, Chongqing 400715; 2College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716; 3Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009) Abstract: 【Objective 】 Four coding regions of chloroplast genome of Citrus and its close relatives were analyzed in an attempt to find suitable DNA barcoding markers for species identification and lay a foundation for further study of non-coding region. 【Method 】 Four chloroplast DNA regions (matK, rpoB, rpoC1 and rbcL ) of 59 Citrus accessions were sequenced, the intergeneric, interspecific, intraspecific genetic distances were calculated, and the phylogenetic tree of all the accessions tested