20101260__蛋白质组概论20101123.ppt

The protein complex network, and grouping of connected complexes. NATURE |VOL 415 | 10 JANUARY 2002 | （四）蛋白质相互作用功能网络 NATURE|VOL 415 | 10 JANUARY 2002 Figure 1 Analysing protein interactions. In the ‘ co- precipitation/ mass spectrometry ’ approach used by Gavin et al.1 and Ho et al.2, an ‘ affinity tag ’ is first attached to a target protein( the ‘ bait ’ ; a). b, Bait proteins are systematically precipitated, along with any associated proteins, on an ‘ affinity column ’ . c, Purified protein complexes are resolved by one-dimensional SDS?PAGE, a technique that involves running an electric charge through the complexes on a gel, so that proteins become separated according to mass. d, Proteins are excised from the gel, digested with the enzyme trypsin, and analysed by mass spectrometry. Databasesearch algorithms (bioinformatics) are then used to identify specific proteins from their mass spectra. 蛋白质相互作用研究的TAP（Tag Affinity Purification）策略 EST cell lysis complex Immunoprecipitate or affinity purify Enzyme digest Nano-ESI-MS Database searching 蛋白质相互作用研究的复合物免疫沉淀研究策略 Shotgun identification of protein modifications from protein complexes and lens tissue PNAS 2002 vol. 99 no. 12 Fig. 1. The identification of glycogen phosphorylase and ovalbumin protein modifications within a simple protein mixture. The protein modifications identified with overlapping peptide coverage are displayed with phosphorylation in blue, acetylation in yellow, and oxidation in red. （五）蛋白质翻译后修饰 Cell 127, 635–648, 3, 2006 细胞信号通路网络的动态磷酸化修饰研究 Complementary Profiling of Gene Expression at the Transcriptome and Proteome Levels in Saccharomyces cerevisiae Molecular Cellular Proteomics 1.4 323 ， 2002 FIG. 1. mRNA abundance ratios versus protein-abundance ratios. The log10 values of the ratio of abundance on the galactose carbon source to the abundance on the ethanol carbon source measured at the mRNA and protein level are plotted against each other for each unique gene product characterized in this