天花粉中总三萜皂苷的含量分析.pdfVIP

29 4 Vo.l 29No. 4 2010 8 Ch inese W ild P lant R esources Aug. 2010 do:i 10. 3969/.j issn. 1006- 9690. 2010. 04. 016 陈 颖, 董孝刚, 孟 丽,孙海燕, 曹银萍 ( , 453003) 目的: 对天花粉中总三萜皂苷含量进行测定, 天花粉质量评价提供依据方法:选用熊果酸 对照品, 5 香草醛- 冰醋酸和高氯酸 显色剂, 以紫外分光光度法测定天花粉中总三萜皂苷的含量结果:标品在 16~ 48g范围内有良好的线性关系(r = 0. 990 5)样品在 10~ 40m in内稳定, 加样回收率 97. 22 ,RSD = 1. 59结 论: 该方法操作简便,快速, 灵敏度高和重复性好, 可用于天花粉及其相关产品的质量控制 天花粉; 总三萜皂苷;分光光度法 : R285. 2 : A : 1006- 9690( 2010) 04- 0061- 03 Study of the Con ten t of Total T riterpenoid s in R adix T richosan th in ChenYing, DongXiaogang, Men Li, SunHaiyan, CaoYinping ( SchoolofLife Science andTechnology, Henan Institute of Science andTechnology, Xinxiang 453003, China) Abs tract Objective: To determ ine the quantity of total triterpenoids in radix trichosanthin. Methods: Total triterpenoidswasdetermined by spectrometry using ursolic acid as atandard and5 vanillin- gla cial acetic and perchloric acid as colcr- developing agents. Results: The content of the standardwas closely correlated to itsopticaldensity in the range of 16~ 48g (r= 0. 9905) with a stabilitywithin 10 ~ 40m in. The average recoverieswas97. 22 , RSD= 1. 59. Conclusion: With smi ple operation, ra pidity, high sensitivity aswell as fine repetition, thismethod can behelpful formonitoring the quality of radix trichosanthin and related products. K ey w ord s radix trichosanthin; total triterpenoids; spectrometry Trichosanthes k irilow ii , [3- 5] Maxmi ., [ 1] , 1 ,,, 1. 1