毕业设计（论文）-玉米蛋白生物大分子有机基质为模板的生物矿化过程及其调控机理研究.doc

摘要 体内骨组织是受细胞高度调节控制生长而成的生物矿化组织，由有机质和无机质两大部分构成。随着组织工程学的发展，利用生物大分子有机基质作为调制生物矿化的模板，设计生物材料并将其用于骨组织的修复取得了较大的进步。 玉米蛋白是一种来自于玉米的含大量疏水氨基酸的蛋白质，Bone tissue in vivo, controlled by cell growth, is a biological mineralized tissue which consists of both organic matter and inorganic matter. With the development of tissue engineering, the use of biological macromolecules as a template for biomineralization modulation, and to design biological materials for bone tissue repair has made great progress.  Zein, came from corn, is a prolamin protein which contains a large number of hydrophobic amino acid content. For its good absorbency and biocompatibility, zein is a new biological material.  This study USES three schemes of corn protein biological macromolecules organic templates biological mineralization processes and regulation mechanism. The first one is by ir, XRD, sem observation and analysis, the research of different PH, add ways such as time and mineralization in different conditions on the influence of the mineralization products. The second option is to use two kinds of methods and amide phosphorylation of corn protein microspheres, then modified with modified corn protein microspheres were of hydroxyapatite mineralization. The third is using different methods of preparation of different size radius of corn protein microspheres, and then use the method, the fluid bubble simulation research corn protein of biomimetic mineralization. 目录 第一章 生物矿化 3 1.1 生物矿化 3 1.1.1 生物矿化材料 3 1.1.2 生物矿化的类型 4 1.1.3 生物矿化的过程 4 1.1.4 生物矿化的机理 5 1.2有机基质诱导生物矿化 7 1.2.1 胶原蛋白 7 1.2.2 丝素蛋白 8 1.2.3 聚乳酸 8 1.3玉米醇溶蛋白 9 1.3.1玉米醇溶蛋白的组成 9 1.3.2玉米醇溶蛋白的构型 10 1.3.3玉米醇溶蛋白的溶解性 10 1.4羟基磷灰石 10 1.4.1羟基磷灰石晶体结构 11 1.4.2生物学特性 12 1.4.3 在生物医学领域的应用 13 1.5 本论文的研究目的与主要内容 14 第二章 玉米蛋白对羟基磷灰石晶体的矿化调控 15 2.1 引言 15 2.2 实验部分 15 2.2.1 材料 15 2.2.2实验步骤 16 2.2.3产物的表征 17 2.3结果与讨论 17 2.3.1工艺条件的影响 17 2.3.2 矿化产物的FT-IR表征 20 2.3.3 矿化产物的形貌 21 2.4 本章小结 22 第三章 改性玉米蛋白对羟基磷灰石晶体的矿化调控 23 3.1 引言 23 3.2 实验部分 24 3.2.1 试剂与仪器 24 3.2.2 模拟体液的制备 24 3.3 结果与讨论