毕业设计（论文）-年产10万吨柠檬酸厂糖化、发酵车间的设计.doc

年产10万吨柠檬酸厂糖化、发酵车间的设计 摘 要 本设计采用薯干原料发酵，只需将薯干磨成粉，加水调浆，直接加入少量α－淀粉酶液化后灭菌、冷却即可接种发酵。制备柠檬酸一般采用晒干的薯干作为原料。其中薯干含水10%-15%、淀粉70%左右、蛋白质6%左右。薯干原料中的蛋白质可作为氮源供菌体生长。薯干原料中含有铁、镁、钾、钙等的无机盐，选用的黑曲霉C0527对这些成分不敏感,故不必对原料做这方面的预处理。本设计采用液体深层好氧发酵、钙盐法提取技术生产柠檬酸。这两种方法都是国内比较流行的生产方法，有着大量的实际经验，易于操作，风险小。 由于本设计为糖化、发酵车间的设计，着重于这两个车间的工艺计算、设备选型。通过全厂物料衡算、车间热量衡算，确定糖化、发酵车间主要设备发酵罐、种子罐、车间管道的设计和选型以及全厂及车间布置。 本设计还包括发酵罐，全厂平面图，车间平面布置图，工艺流程图。 关键词：薯干 深层好氧发酵 黑曲霉 柠檬酸 设备设计和选型 Annual output of 100，000 tons citric acid plant saccharification fermentation，fermentation plant design ABSTRACT The design of raw materials used potato fermentation, simply potato mills, water-paste, adding a small amount of direct α-amylase liquefaction, sterilization, cooling, fermentation can be vaccinated. Preparation of citric acid normally used as raw materials dried potato. Potato moisture content of 10% to 15%, about 70 percent of starch, protein about 6 percent. Potato raw materials in the protein can be used as source of nitrogen for cell growth. Potato raw materials containing iron, magnesium, potassium, calcium of inorganic salt, optional black Aspergillus niger C0527 is not sensitive to these ingredients, it need not do any pretreatment of the raw materials. The design of a good gas liquids deep fermentation, dry calcium citrate extraction technology production. The two methods are more popular domestic production methods, has a great deal of practical experience, easy to operate, the risk small. As the design for the saccharification and fermentation workshop the design, the two workshops focused on the process, the selection of equipment. Through the whole plant material balance, energy balance workshop, identified glycosylation, fermentation workshop major equipment fermenter, seed tanks, pipelines and workshops in the design and selection. And the whole plant and facility layout. The design also includes Fermentor, The factory plan, Shop floor plan, Flow Chart. Key words: Potato Deep-seated aerobic fermentation Aspergillus n