两性离子表面活性剂BS_12与DNA作用的共振光散射光谱_陈展光.pdfVIP

25, 10 Vol125, No1 10, pp1680- 1683 2 0 0 5 1 0 Spectroscopy and Spectral Analysis October, 2005 BS-12 DNA 1, 2 1 1 1 1 1 陈展光 , 廖栩泓, 韩雅莉 , 杨英宜, 卢 峰, 丁卫锋 11 , 515063 21 , 410083 DNA pH 13 , DNA ( BS- 12) 388 nm , , DNA fsDNA ctDNA 01 25~ 1210 Lg#mL- 101 25~ 111 0 Lg# mL- 1, 01 15 01 16 ng#mL- 1 DNA , ( RLS) ; ; ( BS- 12) ; DNA : O64411 : A : 1000-05 3(2005) 10- 1680-04 Agilent 8453 - ( ) ; pHS-3 ( ) DNA : DNA( fsDNA, Sigma ) DNA( ctDNA, , ) 0~ 4 e , 501 0 mg#L- 1 [ 1, 2] [ 3, 4] (reso- BS- 12 : BS- 12( ) 1% nance light scattering, RLS) Tris-HCl (pH 13) [ 5-7] , , 112 10 mL 110 mL , 014 mL / 0 BS- 12 , [ 8] , , , , K = K ex em , , 388 nm RLS , [ 8] ( ) 51 0 nm DNA , ( dodecyl dimethyl betaine,