袋鼠源铜绿假单胞菌分离鉴定.pdfVIP

第32 卷 第2 期 中 国 预 防 兽 医 学 报 Vol. 32 ，No.2 2010 年 2 月 Chinese Journal of Preventive Veterinary Medicine Feb. 2010 袋鼠源铜绿假单胞菌分离鉴定 * 赵 耘 ，杜昕波，李伟杰，陈 敏，康 凯，陈永林 ( 中国兽医药品监察所，北京 100081) 摘 要：为确定袋鼠死亡原因，本研究采用Biolog 快速鉴定系统、16S rRNA 序列分析以及传统细菌鉴定方 法对2 株分离自北京动物园袋鼠肺脏的菌株进行形态学、培养特性、生化特性、小鼠致病性、系统发育分析等生 物特性的鉴定和分析，结果表明2 菌株均为铜绿假单胞菌，对昆明小鼠有强致病性。系统发育分析结果表明2 株 袋鼠源铜绿假单胞菌 16S rRNA 序列与ATCC 10145 模式株差异很小，同源性分别为99.9 %和 100 %，并且位于 系统发育树的同一分支。 关键词：铜绿假单胞菌；Biolog 快速鉴定系统；16S rRNA；系统发育分析 中图分类号：S852.6 文献标识码：A 文章编号：1008-0589(2010)02-0139-03 Isolation and identification of P s eudom ona s a erug inos a in kangaroo * ZHAO Yun , DU Xin-bo, LI Wei-jie, CHEN Min, KANG Kai, CHEN Yong-ling (China Institute of Veterinary Drug Control, Beijing 100081, China) Abstract: The biological characteristics of two bacterial strains isolated from kangaroo were identified, based on the morphol- ogy observation, cultural character, biochemical property, pathogenicity and phylogenetic analysis by Biolog microbes identifica- tion system, 16S rRNA gene sequence analysis and the other classical methods. The results showed that the two bacteria isolates were Pseudomonas aeruginosa, and were pathogenic to mice. The results of phylogenetic analysis showed that the homology of two isolates compared with ATCC 10145 (type strain) were 99.9 % and 100 %, and they were in the same evolution branch. Key words : p seudomonas aeruginosa; Biolog microbes identification system; 16S rRNA; phylogenetic analysis 铜绿假单胞菌(Pseudomonas aeruginosa)又称绿脓 1 材料和方法 杆菌，属于假单胞菌科(Pseudomonadceae) 、假单胞 菌