小于胎龄儿大鼠ghrelin水平变化及其与GH／IGF-1生长轴的关系.docVIP

小于胎龄儿大鼠ｇｈｒｅｌｉｎ水平变化及其与ＧＨ／ＩＧＦ-１生长轴的关系 　　作者：沈振宇，杜敏联， 黄越芳，苏喆， 邓红株 作者单位：中山大学附属第一医院儿科， 广东 广州 510080 　　【摘要】 探讨ghrelin在小于胎龄儿发生矮身材中的作用。【方法】 采用母鼠妊娠期饥饿法，建立SGA动物模型。根据新生鼠及其生后第4周时体质量和/或身长分为有生长追赶SGA组、无生长追赶SGA组和正常对照组即适于胎龄儿(AGA)鼠组;幼鼠每周测量体质量和鼻肛长。4周龄时测定24 h尿GH排泄率 。分别于4周、8周和12周测定血清ghrelin、IGF-1、IGFBP3浓度。【结果】 正常SD雄性大鼠在生后4周和12周的血ghrelin水平分别为(1.6 ± 0.3) ng/mL和(1.8 ± 0.5) ng/mL，无统计学意义(P 0.05)。4周龄雄性SGA鼠血ghrelin水平显著高于正常大鼠，但24 h尿GH排泄量无相应升高，且两者间无相关性;但ghrelin水平与血IGF-1、血IGFBP3水平呈有意义的负相关，分别r = -0.333，P = 0.031;r = -0.338， P = 0.028。12周龄有生长追赶组SGA大鼠的血ghrelin水平(1.4 ± 0.5) ng/mL与对照组比较明显下降(P 0.05)，SGA组血IGF-1 和IGFBP3水平显著降低，且无生长追赶组更低(P 0.05)。24 h尿GH排泄量各组间未见差异(P 0.05)。【结论】 SGA大鼠可能存在ghrelin抵抗并与GH抵抗汇同加重了对SGA生长的损害。 　　【关键词】 ghrelin; 小于胎龄儿; 生长追赶; 胰岛素样生长因子-1 　　Ghrelin Level Variation and Its Relation with GH/IGF-1 Growth Axis in Smallor Gestational Age Rats　SHEN Zhen-yu， DU Min-lian， HUANG Yue-fang， SU Zhe， DENG Hong-zhu　　Department of Pediatrics， The First Affiliated Hospital， Sun Yat-sen University， Ghangzhou 510080， China　Abstract： 【Objective】 To observe the ghrelin level variation and its relation with GH/IGF-1 growth axis in small for gestational age (SGA) rats and to explore the role of ghrelin as a medium and regulation of metabolism and growth in SGA rats. 【Methods】 Establishment of the SGA animal model made by starvation method in pregnant rat. Experiment Grouping： ① Group C： Normal matched control; ② Group S1： SGA group with growth catch-up; ③ Group S2： group without growth catch-up. The body weight and nose anuses length were measured weekly. 48-hour urines were collected for detecting the excretory rate of GH when the young rat were 4 weeks old. The serum of group C was sampled on the 4th， 8th， and 12th week respectively while the serum of group S1 and S2 were collected on the 4th and 12th week， and then the concentration of ghrelin， insulin， IGF-1， and IGFBP3 were evaluated. 【Results】 The ghrelin level remains invariant in normal SD male rats of different stages after birth 4 week