接种抗性菌对植株体内菌群的影响.pdfVIP

Methylobacterium-Induced Endophyte Community Changes Correspond with Protection of Plants against Pathogen Attack 1 2 ¨ 1 3 ¨ 1 Pavlo Ardanov , Angela Sessitsch , Hely Haggman , Natalia Kozyrovska , Anna Maria Pirttila * 1 Department of Biology, University of Oulu, Oulu, Finland, 2 Bioresources Unit, AIT Austrian Institute of Technology GmbH, Tulln, Austria, 3 Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine Abstract Plant inoculation with endophytic bacteria that normally live inside the plant without harming the host is a highly promising approach for biological disease control. The mechanism of resistance induction by beneficial bacteria is poorly understood, because pathways are only partly known and systemic responses are typically not seen. The innate endophytic community structures change in response to external factors such as inoculation, and bacterial endophytes can exhibit direct or indirect antagonism towards pathogens. Earlier we showed that resistance induction by an endophytic Methylobacterium sp. in potato towards Pectobacterium atrosepticum was dependent on the density of the inoculum, whereas the bacterium itself had no antagonistic activity. To elucidate the role of innate endophyte communities in plant responses, we studied community changes in both in vitro and greenhouse experiments using various combinations of plants, endophyte inoculants, and pathogens. Induction of resistance was studied in several potato (Solanum tuberosum L.) cultivars by Methylobacterium sp. IMBG290 against the pathogens P. atrosepticum, Phytophthora infestans and Pseudomonas syringae pv. toma