氨基酸突变对蛋白质稳定性的影响预测及其在线工具_欧阳玉梅.pdfVIP

免疫学杂志 年 月 第 卷 第 期 2013 11 29 11 IMMUNOLOGICAL JOURNAL Vol. 29 No. 11 Nov. 2013 · · 997 文章编号 （ ） ·综 述· [ ]1000-8861 2013 11-0997-05 氨基酸突变对蛋白质稳定性的影响预测及其在线工具 * 欧阳玉梅 ，方若森 摘 要 确定氨基酸突变对蛋白质稳定性的影响对于分子水平的疾病机理理解和新蛋白质设计是十分重要的。 这项研究也有 [ ] 助于药物学上的为提高蛋白质药物的稳定性而对其进行的改造与重组以及免疫学上的疫苗设计。 实验测定费时费力且代价昂贵。 计算方法已用来预测突变的影响和阐明其潜在的生物学机制。 本文阐述预测残基突变对蛋白质稳定性影响的两类方法：使用能量 函数直接计算法和机器学习方法；介绍 个最新在线预测工具并说明面临的挑战与未来的方向。 15 关键词 点突变；蛋白质稳定性；解折叠自由能的改变；预测工具 [ ] 中图分类号 ， 文献标识码 [ ] TP39 Q78 [ ] A Prediction of protein stability changes upon mutations and its web-based tools OUYANG Yumei1,*, FANG Ruosen2 1. Department of Liberal Arts Science, Xinjiang Career Technical College, Kuitun 833200, China; 2. Academic Administration, Xinjiang Career Technical College, Kuitun 833200, China *Corresponding Author: OUYANG Yumei, E-mail: fang9352@ [Abstract] It is very important to determine protein stability changes upon mutations for understanding the molecular underpinnings of diseases and designing of new proteins. It is also helpful for the research of modifying and recombinant of protein agents which increases its stability on pharmacology and for immunological vaccine design. Experimental studies on the protein stability changes upon mutations are often laborious, time-consuming, and costly. In recent years, computational methods are used to predict the effects caused by mu