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Microarray expression analysis of epithelial ovarian cancer with distinct differentiation.pdf

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Microarray expression analysis of epithelial ovarian cancer with distinct differentiation.pdf

维普资讯 JournalofMedicalCollegesofP A 2007：22(6) 373 M icroarrayexpressionanalysisofepithelialovariancancerwithdistinct differentiation LIYan—hong～ (李艳红)，ZHANGWei(张伟)，ZHUShao—jun (朱少君)，YINGuo—wu(尹国武)，YAN Kai—lin(闫凯麟)，ZHAOHong—xi(赵宏喜) Departmentof ObstetricsandGynaecology，TangduHospital，FourthMilitaryMedicalUniversity，Xi’an 710038，China Departmentof Pathology。TangduHospital，FourthMilitaryMedicalUniversity，Xi’an710038，China [Abstract] Toidentifygeneexpressionprofilinginepithelialovariancancerandtoexploreitscorrelation withhistopathologycharacterizationandprognosis．Geneexpressionprofilesweregeneratedfrom 10hu— manovarianfrozentissuespecimensusingAgilentHuman 1A microarrays．Strikingly，cleardifferencesof geneexpressionpatternswereobservedinovariancancerascomparedtonormaltissues．Uniquegenepro— fileswereobservedinmoderatelyandpoorlydifferentiatedepithelialovariancancer．Itisconcludedthat differenthistopathologycharacterization likelyexistsextensivemolecularheterogeneity． [Keywords] ovariancancer；microarray；differentiation Epithelialovariancanceristheleadingcauseof ticsofallspecimenswere judged by the same death from gynecologicalcancerinwomen．Despite pathologist．Threemoderately differentiated and 2 advances in molecular biology， surgery， and poorly differentiated cancers were used in this chemotherapy，ovariancancerremainsachallenging study．TotalRNA isolation and purification was diseasetomanageandlong—term survivalrateshave performedaccordingtotheinstuction (RNeasyMini hardlychangedinthelast3decades[．The 5-year Kit50，Qiagen)．Thequality oftotalRNA was overallsurvivalisonly 25 [．Yet，themolecular checked