蒸汽爆破玉米秸秆高底物浓度酶解和乙醇发酵.pdfVIP

中国可再生能源学会2011 年学术年会论文（生物质能专业） 蒸汽爆破玉米秸秆高底物浓度酶解及乙醇发酵 * * 门珣 郝学才 张宜 杨秀山 田沈** 北京市海淀区西三环北路105 号首都师范大学生命科学学院 摘要：本研究使用可高效发酵生产乙醇并具有一定对抑制剂耐受性的酵母菌株对蒸汽爆破预处 理后的玉米秸秆进行了酶解及发酵研究，运用了两种不同的反应方式并进行对比。使用滚动式反 应装置进行高底物浓度的酶解及发酵，可在酶解反应中得到85.2g/L 葡萄糖，纤维素转化率可达 到84.1%，发酵后乙醇浓度为39.1g/L，总转化率为75.6%。 关键词：玉米秸秆；高底物浓度；乙醇发酵 EnzymaticHydrolysisandEthanolFermentationwithHighSubstrate EnzymaticHydrolysisandEthanolFermentationwithHighSubstrate EEnnzzyymmaattiiccHHyyddrroollyyssiissaannddEEtthhaannoollFFeerrmmeennttaattiioonnwwiitthhHHiigghhSSuubbssttrraattee ConcentrationUsingSteamExplosionPretreatedCornStover ConcentrationUsingSteamExplosionPretreatedCornStover CCoonncceennttrraattiioonnUUssiinnggSStteeaammEExxpplloossiioonnPPrreettrreeaatteeddCCoorrnnSSttoovveerr MENXun,HAOXuecai, ZHANGYi,YANGXiushan,TIANShen* MENXun,HAOXuecai, ZHANGYi,YANGXiushan,TIANShen* MMEENNXXuunn,,HHAAOOXXuueeccaaii,,ZZHHAANNGGYYii,,YYAANNGGXXiiuusshhaann,,TTIIAANNSShheenn** College of Life Science, Capital Normal University, Beijing 100048, China Abstract: Abstract: AAbbssttrraacctt:: A research on enzymatic hydrolysis and ethanol fermentation using steam explosion pretreated corn stover with an efficient and toxin-tolerant yeast stain was performed. Two modes of process with different equipments were contrasted. In the roller bottle equipment, the glucose concentration in enzymatic hydrolysis process could reached 85.2g/L, corresponding to 84.1% of the theoretical max; after the fermentation process, an ethanol concentration of 39.1g/L was obtained, and the total conversion from cellulose to ethanol was 75.6%. Key words: Key words: KKeeyyww