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The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes.pdf
维普资讯
· 34 · JReprodMed2007Vo1.16No.S1
Theinfluenceofmacrophageco—culturetothefertilityrateandblastocyst
formationrateofthemouse’soocytes
ChenMei(陈梅 ) ,SunZheng-yi(~,]、正怡),YuQi(郁琦)¨,HeFang-fang( 方方)
objective:Toinvestigatetheeffectofco—culturewithperitonealmacrophagetothefertilityrateandblastocyst
formationrateofthemouse’soocytes.
Methods:A totalof15 KunMing miceaged 10—14 weekswere undergoneovulation induction and oocytes
collection.Theoocyteswererandomlydividedintothreegroups.Thefirstgroupwasthecontrolgroupandcultured
withoutmacrophage:thesecondgroup (experimentalgroup1)wasco—culturedwithmacrophagesrightafteroocytes
collection;thethirdgroup (experimentalgroup2)wasculturedaloneafteroocytesretrieval,andco—culturedwith
macrophagesfrom 8cellstage.
Results:1.Thefertilityrateincontrolgroup (91.0 )andexperimentalgroup2(93.7V00)wassignificantly
higherthanthatinexperimentalgroup1(72.OH,PO.001).2.Blastocystformationrateofthefertilizedoocytes
intheexperimentalgroup2(78.2V00)wasmuchhigherthancontrolgroup (60.3 ,PO.001).3.Blastocyst
formationrateoftheoocytesinexperimentalgroup2(73.2V00)wasmuchhigherthancontrolgroup(54.8 )and
experimentalgroup1 (50.0 ),whichwerestatisticallysignificant.
Conclusions:1.Co—culturewith peritonealmacrophageshad anadverseinfluenceonthefertilizationprocess.
2.Peritonealmacrophagescouldenhancethemouseembryonicdevelopmentand increasetheblastocystformation
rate.
Keywords:Invitrofertilization;Macrophage;Co-culture;Mouseoocyte;Blastocystformation
CLCnumber:Q492.6 Documentcode:A ArticleID:1004—3845(2007)S1—0034一O5
evidently.Thepurposeofourstudyisto investi—
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