The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes.pdfVIP

The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes.pdf

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The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes.pdf

维普资讯 · 34 · JReprodMed2007Vo1.16No.S1 Theinfluenceofmacrophageco—culturetothefertilityrateandblastocyst formationrateofthemouse’soocytes ChenMei(陈梅 ) ,SunZheng-yi(~,]、正怡),YuQi(郁琦)¨,HeFang-fang( 方方) objective:Toinvestigatetheeffectofco—culturewithperitonealmacrophagetothefertilityrateandblastocyst formationrateofthemouse’soocytes. Methods:A totalof15 KunMing miceaged 10—14 weekswere undergoneovulation induction and oocytes collection.Theoocyteswererandomlydividedintothreegroups.Thefirstgroupwasthecontrolgroupandcultured withoutmacrophage:thesecondgroup (experimentalgroup1)wasco—culturedwithmacrophagesrightafteroocytes collection;thethirdgroup (experimentalgroup2)wasculturedaloneafteroocytesretrieval,andco—culturedwith macrophagesfrom 8cellstage. Results:1.Thefertilityrateincontrolgroup (91.0 )andexperimentalgroup2(93.7V00)wassignificantly higherthanthatinexperimentalgroup1(72.OH,PO.001).2.Blastocystformationrateofthefertilizedoocytes intheexperimentalgroup2(78.2V00)wasmuchhigherthancontrolgroup (60.3 ,PO.001).3.Blastocyst formationrateoftheoocytesinexperimentalgroup2(73.2V00)wasmuchhigherthancontrolgroup(54.8 )and experimentalgroup1 (50.0 ),whichwerestatisticallysignificant. Conclusions:1.Co—culturewith peritonealmacrophageshad anadverseinfluenceonthefertilizationprocess. 2.Peritonealmacrophagescouldenhancethemouseembryonicdevelopmentand increasetheblastocystformation rate. Keywords:Invitrofertilization;Macrophage;Co-culture;Mouseoocyte;Blastocystformation CLCnumber:Q492.6 Documentcode:A ArticleID:1004—3845(2007)S1—0034一O5 evidently.Thepurposeofourstudyisto investi—

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