甘蓝型油菜(Eru+CMS)和红菜薹回交四代(BC4)特性研究.pdfVIP

甘蓝型油菜(Eru 性研究 吴红美，谭远宝，徐跃进。，万正杰 (华中农业大学园艺林学学院蔬菜系．国家蔬菜改良中心华中分中心．园艺植物生物学教育部重点实验室．武汉430070) 摘要：以新型甘蓝型油菜细胞质雄性不育系(EruCMS)为不育源．通过远缘杂交结合faI交的方法．并 运用组织培养、单株选择、异地加代种植的手段已成功将雄性不育基因转到红菜謦卜．L二获得其与红菜薹的回 交四代(BC．)。对I廿1交明代进行植物学性状iJ；54食、染色体数fj观察、育性调查以及AFLP分析等研究．研究表 明：回交四代植物学性状基本和红菜．甍表现一致．同时苗期遇低温叶片不黄化．不育性稳定．蜜腺正常。不育度 和不育株率均为100％；染色体数目和AFLP分析也为回交四代趋向于红菜薹提供依据；说明回交四代已基 本具备成为红菜蕞细胞质雄性不育系的条件。 关键词：甘蓝型油菜；红菜薹；回交四代(BC．)；特性分析 onCharacterofBackcross Brassicana Study Generations(BC4)ofpus and Cai—tai Purple WU Yuan·bao，XU Hong—mei·TAN Yue-jin‘·WANZheng—jie Horticultureand Center (Collegeof Forestry，HuazhongAgric。ulturalUniversity；Nationalfor HorticulturalPlant vegetableImprovement(CentralChina)；KeyLaboratoryof Biology，Ministry Education，Hubei，Wuhan430070) of new of male line used Abstract：A ofBrassica CMS)wasas typecytoplasmicsterility napus(Eru sterilesource．The male were transferredto Cai·tai cytoplasmicsterilitygenes successfully Purple by distant andbackcrossandBC4wereobtained suchmethodsas：tissueculture， hybridization through individualselectionand variouscharactersthe wereinvesti． allopatricpropagation．Here of hybrid resultsshowedthat werealmost similarto Cai-taiand BC4 gated．The plants morphoIogicallyPurple theirIeavesof wouldnotbecomeetiolationwhentheenvironment was seedlingstage tempreture was was of rate and ofsterile low．Steri