C1Q%2fTNF相关蛋白家族4(CTRP4)%3a一个新炎症抑制因子.pdfVIP

C1Q/ TNF 相关蛋白家族 4 （CTRP4 ） ：一个新的炎症抑制因子 罗 阳,马 壮,谭伟峰,王兰兰,尹 昂,吴晓彤， 黄 河,王 露,马大龙 （北京大学人类疾病基因研究中心，北京 100191 ） 摘要：目的：研究CTRP4 在炎症中的作用及机制。方法：使用 RT-PCR 、ELISA 、免疫印迹实验等，在体外检测 CTRP4 在炎症中的表达变化和对炎症的抑制作用，通过人 CTRP4 转基因鼠研究其在体内的作用，即在 LPS 诱导 的内毒素休克和DSS 诱导的急性结肠炎过程中 CTRP4 的作用。结果：在炎症中CTRP4 的表达明显上调；体外重 组 CTRP4 蛋白可以抑制炎症因子的产生；可以降低 LPS 刺激的 THP1 细胞和 RAW264.7 细胞 TNF- α分泌，并降 低 p-Erk1/2 的磷酸化水平。体内实验表明，人CTRP4 转基因鼠可以明显减轻 DSS 诱导的结肠炎症状，使炎症因 子产生更少；可以延长 LPS 诱导的内毒素休克小鼠的生存期。结论：CTRP4 是一个新的炎症抑制因子，在固有 免疫细胞介导的炎症反应中有重要的负向调控作用。 关键词：人体免疫学；细胞因子；结肠炎；CTRP4 CTRP4：A New Inflammatory Inhibit Factor LUO Yang, MA Zhuang, TAN Weifeng, WANG Lanlan, YIN Ang, WU Xiaotong, HUANG He, WANG Lu, MA Dalong (Peking University Center for Human Disease Genomics, Beijing 100191, China) Abstract: Subject: To investigate the role of CTRP4 during inflammatory response. Methods: RT-PCR, ELISA and immunobloting were used to determine the regulation of expression of CTRP4, and the role of CTRP4 during inflammation. CTRP4 transgenic mice models of DSS induced colitis and LPS induced endotoxin shock were used to investigate the function of CTRP4 in the process of inflammation in vivo. Results: Production of CTRP4 was increased during inflammatory response, and co-stimulating RAW264.7 and THP1 cells with rhCTRP4 and LPS could inhibit LPS-induced production of TNF-α and p-Erk1/2 in vitro. Furthermore, CTRP4 transgenic mice displayed lower level of colitis activity and produced less pro-inflammation cytokines. CTRP4 transgenic mice were also resistant to LPS-induc