载药纳米羟基磷灰石%2f聚酰胺复合支架制备和表征.pdfVIP

第十二届全国青年材料科学技术研讨会（2009年11 月27-30） 5 特种功能材料 载药纳米羟基磷灰石/聚酰胺复合支架 制备与表征 黄棣，左奕，邹琴，李吉东，沈娟，李玉宝 (四川大学分析测试中心，纳米生物材料研究中心，四川 成都 610064) 摘 要：利用相转移法制备了纳米羟基磷灰石/聚酰胺 66 (n-HA/PA66)复合多孔支架。采用浸涂法在多孔支 架基体表面制备含盐酸小檗碱的壳聚糖缓释涂层，并使用扫描电镜(SEM)和材料力学试验机对多孔支架浸 涂前后的形貌和力学性能进行了表征。研究了载药 n-HA/PA66 复合多孔支架在磷酸盐缓冲溶液(PBS) 中的 药物释放行为。结果显示，多孔支架具有较为理想的孔隙结构和贯通性，载药壳聚糖溶液浸涂后，其力学 强度有明显提高。药物释放结果表明盐酸小檗碱能实现持久、缓慢的释放。 关键字：生物材料；纳米羟基磷灰石；聚酰胺 66；多孔支架；壳聚糖；盐酸小檗碱；药物释放 Preparation and characterization of drug delivery system based on nano-hydroxyapatite/polyamide66 porous scaffold HUANG Di, ZUO Yi, ZOU Qin, LI Jidong, SHEN Juan, LI Yubao (Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan 610064) Abstract: Nano-hydroxyapatite/polyamide66 (n-HA/PA66) porous scaffolds were fabricated by phase inversion method. Chitosan containing antibiotic berberine was used to coat the scaffolds to form the drug delivery system. The morphology, mechanical property of the scaffolds before and after coating were obtained by using scanning electron microscopy (SEM) and mechanical testing machine. The drug release behavior was investigated in the phosphate buffered saline (PBS) solution at predetermined periods of time. The results show that the scaffolds exhibit open and interconnected porous structure. The mechanical properties of the scaffolds after coating were improved significantly. The drug release results indicate that the drug could be released stably and slowly. Key words: biomaterial; nano-hydroxyapatite; polyamide66; porous scaffold; chitosan; berberine; drug release 0 引言 组织工程用多孔支架因其高孔隙率、合适的孔径及优良的表面性能，在骨组织损伤修复 中得到了广泛的研究。但在骨缺损修复过程中往往会出现细菌感染的问题，在骨再生过程中， 细菌感染可能长期存在[1] 。传统的方法采用药物注射以减轻感染，但是相对较高的血药浓度 会对人体产生一系列的副作用。因此，在植入支架材料中引入抗菌药物抑制局部感染为解决 这一问题提供了新思路。 壳聚糖(chitosan, CS)