沼泽红假单胞菌菌体富硒的生产工艺研究.pdfVIP

沼泽红假单胞菌菌体富硒的生产工艺研究 1 1 1 1 1 冯波 ,赵红卫 ,方为茂 昌宇奇 熊莹 1 ( 四川大学化学工程学院，四川 成都 610065) 摘要：从混合菌种中分离纯化出沼泽红假单胞菌（Rhodopseudomonas palustris），通过耐硒能力筛选研究表明，沼 泽红假单胞菌的耐硒能力较好，以浓度梯度法对其驯化，达到富硒菌株并进行富硒试验。富硒培养条件为：温度 30～35 ℃，pH 值7.0，光照强度1000Lx。当硒浓度16 μg/ml 时，硒对菌株的生长基本没有抑制，表现出较好的耐硒水平；而硒 浓度超过 16 μg/ml 后，开始抑制菌株的生长。通过驯化使菌株在硒浓度45 μg/ml 下生长，该菌株的富硒能力可达704.2 μg/g 干菌。 关键词：沼泽红假单胞菌；富硒；驯化 中图分类号：Q931 文献标识码：A 文章编号： Study on Rhodopseudomonas palustris Enriching Selenium 1 1 1 1 1 Feng Bo ,Zhao Hongwei , Fang Weimao Chang Yuqi Xiong Ying (1.School of Chemical Engineering Sichuan University, Chengdu 610065,Sichuan,China) Abstract: Rhodopseudomonas palustris (RP) was isolated from the mixed germ. The Se-enriched strains was obtained through screening and domesticated by incresing the Se-concentration. The cultivated condition is at pH 7.0, 30~35 ℃ and 1000lx. During the experiment of enriching selenium, the results showed that Rhodopseudomonas palustris had a great ability of Se-tolerance. With the Se concentration of 16µg/ml, RP growed best, while the concentration overed 16µg/ml, Se had inhibition effects on the growth of RP. The former was domesticated by increasing the Se-concentration from 15µg/ml, and now the strain has growed well in the Se-concentration of 45µg/ml and the content of selenium has reached 704.2µg/g in dry strains. Key words: Rhodopseudomonas palustris; enriching selenium; domestication 沼泽红假单胞菌的用途很多，可用于水质净 引 言 化、污水处理、饲料级微生物添加剂等。在废水处 理方面，由于它有能忍耐高深度的有机废水和较强 沼泽红假单胞菌(Rhodops