大蒜素对胃上皮细胞Cx37Cx43mRNA表达及细胞间隙连接通讯功能的影响.docVIP

大蒜素对胃上皮细胞Cx37、Cx43 mRNA表达及细胞间隙连接通讯功能的影响 　　作者：罗小玲，徐灿霞△，胡 琳 作者单位：410013 湖南长沙，中南大学湘雅三医院消化内科(△通迅作者) 　　【摘要】目的 观察体外实验中大蒜素对胃上皮细胞系MGC-803 Cx 37 mRNA、Cx 43 mRNA表达及细胞间隙连接通讯功能的影响。方法 终浓度为3 Μg/ml、6 Μg/ml、9 Μg/ml、12Μg/ml大蒜素分别加入胃上皮细胞MGC-803培养 24 h、48 h、72 h，同时设立不加药物的阴性对照，用RT-PCR法检测细胞Cx 37 mRNA、Cx 43 mRNA表达，LY染料传输方法检测细胞间隙连接通讯功能。结果 MGC-803细胞Cx 37 mRNA有表达，Cx 43 mRNA无表达，GJIC功能弱，加入不同浓度大蒜素后，Cx 37 mRNA表达和细胞间隙连接通讯功能增强，Cx 37 mRNA表达随大蒜素浓度和培养时间的增加而增强(均P0.05)，Cx 43 mRNA仍无表达。 结论 大蒜素在转录水平上调胃上皮细胞MGC-803 Cx 37基因的表达，改善细胞间隙连接通讯功能。 　　【关键词】 大蒜素;胃上皮细胞;细胞间隙连接通讯功能 　　Effects of allicin on expression of connexin 37，connexin 43 mRNA and gap junction intercellular communication of gastric epithelial cells 　　LUO Xiao-Ling，XU Can-Xia，HU Lin.Department of Gastroenterology，The Third Xiangya Hospital of Central South University，Changsha 410013，China 　　[Abstract] Objective To observe the effects of allicin on the expression of connexin 37 mRNA，connexin 43 mRNA and gap junction intercellular communication of gastric epithelial cells MGC-803 in vitro.Methods MGC-803 cell were interfered with various concentration allicin(3，6，9，12 Μg/ml ) for 24，48 and 72 hours respectively and control groups with no intervention.RT-PCR method was applied to detect the expression of connexin 37 mRNA，connexin 43 mRNA in cells.Lucifer Yellow Scrape-loading and dye transfer method was applied to detect gap junction intercellular communication.Results MGC-803 cell expressed Cx 37 mRNA，it didnt express Cx 43 mRNA.Cell gap junction intercellular communication was weak in MGC-803 cell.The expression of Cx 37mRNA and gap junction intercellular communication was enhanced after interfered with variable dose allicin.The expression of Cx 37mRNA was up-regulated with the increasement of concentration and action time of allicin (P0.05).There was still no Cx 43 mRNA expression in MGC-803 cell.Conclusion Allicin can up-regulate expression of Cx 37 in MGC-803 cell at the transcription level，increase GJIC function. 　　[Key word