杜仲叶片衰老过程中的细胞程序性死亡.doc

杜仲叶片衰老过程中的细胞程序性死亡 Programmed Cell Death during Leaf Senescence in Eucommia ulmoides Oliv.* 生命科学学院 植物分子与发育学系 98级 江枫 Abstract Leaf tissues from Eucommia ulmoides Oliv. trees were used to study the mechanism of programmed cell death (PCD) during leaf senescence. Here, we show that DNA ladders were detected by gel electrophoresis only in senescent leaves. DNA fragmentation and nuclear DNA condensation were further confirmed by using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method. A 20 kDa DNase was detected only in senescent leaves, which s