荧光原位杂交法和免疫组织化学法检测乳腺癌c.docVIP

荧光原位杂交法与免疫组织化学法检测乳腺癌cerbB2表达的比较 　　作者：盛薇,车向明,单涛,樊林　　作者单位：西安交通大学医学院第一附属医院普外科，陕西西安 　　【摘要】目的 比较免疫组织化学法(IHC)和荧光原位杂交法(FISH)检测乳腺癌cerbB2状态的一致性。方法 选取乳腺癌石蜡标本共50例，其中以IHC法检测cerbB2蛋白表达(+)10例、()20例、()20例。以FISH法检测上述乳腺癌细胞cerbB2基因扩增情况,分析比较两种方法差异性与相关性。结果 在IHC检测乳腺癌cerbB2(+)和()时，FISH检测结果与其具有较好的一致性，总符合率为89.2%。在IHC检测cerbB2()时，FISH结果与其一致性较差，符合率仅为35.3%。结论 IHC可以作为初步筛查cerbB2表达的首选方法;IHC检测乳腺癌cerbB2(+)和()时与FISH有良好的一致性。因IHC检测cerbB2表达()时与FISH结果的一致性较差,这类患者在应用赫赛汀治疗时应做FISH确诊。 　　【关键词】 乳腺癌,cerbB2,免疫组织化学技术,荧光原位杂交技术 　　ABSTRACT: Objective To compare the consistency of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in detecting cerbB2 status in breast cancer tissues. Methods A total of 50 breast cancer paraffin embedded samples were selected, of which there were 10 cases of cerbB2 protein expression (+), 20 cases of () and 20 cases of (). FISH was used to assess the amplification of cerbB2 gene, and SPSS 13.0 software was employed to analyze the difference and consistency between the two methods. Results IHC and FISH methods had a good consistency when detecting cerbB2 (+) and () expressions in breast cancer tissues, with the coincidence rate of 89.2%. However, when IHC was used to test cerbB2 (), the result of FISH was quite different, with the coincidence rate of only 35.3%. Conclusion IHC is a preliminary method to detect cerbB2 status in breast cancer. IHC and FISH methods have a good consistency in detecting cerbB2 (+) and () status in breast cancer tissues. As detection of cerbB2 () with IHC has a different result from FISH, such patients should receive FISH confirmation for herceptin therapy. 　　KEY WORDS: breast cancer; cerbB2; immunohistochemistry; fluorescence in situ hybridization 　　乳腺癌已成为威胁女性健康的重要危险因素，如何最大限度地减少乳腺癌对广大妇女的严重危害，一直是研究的热点。目前cerbB2基因/蛋白被公认为乳腺癌的标志物，cerbB2过表达的患者容易复发、转移，且预后差、生存率低。 　　2002年美国FDA批准了一种全新的药物Herceptin，该药能有效地改善乳腺癌cerbB2过表达患者的生存。但在该药物的临床应用中，对cerbB2表达的检测至关重要。目前广泛应用的免疫组织化学法