野生牛蒡根提取物的抑菌作用研究.pdfVIP

, Journal of AnhuiA gri. S .i 2010, 38( 33) : 18765- 18767 姜丽 卢瑶 1 1 2 弥春霞 , 姜 明 , 任玉兰 ( 1. , 157012; 2. , 157012) [目的]研究野生牛蒡(A rctum lapp a L. )根提取物的抑菌作用, 为其开发应用提供理 依据[方法]采用琼脂打孔扩散法和最低 抑菌浓度法研究了野生牛蒡根水提物和 70%乙醇提取物的抑菌活性[结果] 2种提取物对供试金黄色葡萄球菌( S tap hy lococcus au- reus. )枯草杆菌(B acillus subtilis )普通变形杆菌(P roteus vulgar is) 和大肠杆菌(Escherich ia coli) 都具有抑菌效果70%乙醇提取物的抑 菌效果优于水提取物, 其对金黄色葡萄球菌枯草杆菌的最低抑菌浓度皆为 31. 25 mg/m,l 对普通变形杆菌的最低抑菌浓度为 62. 50 mg/m,l 对大肠杆菌的最低抑菌浓度为 250. 00 mg/m l; 水提物和乙醇提取物对啤酒酵母(Saccharomy ces erev isiae) 和白色念珠菌(M on ilia albican )均未表现出抑菌作用[结 ]野生牛蒡根水提物和乙醇提取物都具有较好的抑制细菌的作用, 但无抑制酵母菌的作用, 70% 乙 醇提取物抑制细菌的效果优于水提物 牛蒡根; 抑菌作用; 提取物; 琼脂打孔法; 最低抑菌浓度 + S 567. 23 9 A 0517- 6611( 2010) 33- 18765- 03 Study on theAntibacterialEffect ofVarious SolventExtracts fromW ildA rctium lappa L. Roots MIChun-xia et al ( College of L ife S ien e and Te hnology, Norm al College ofMudanjiang, Mudanjiang, H eilongjiang 157012) Abstract [Ob je tive] The ami was to study the antiba ter ial effe t of var ious solvent extra ts from w ildA rctium lapp a L. roots so as to prov ide theoreti al basis for its development and utilization. [M ethod] Them ethod of m inmi um inhibitory on entration(M IC) and agar- well diffusion were adopted to dete t the antmi i robial a tiv ity of aqueous and 70% ethanol ex tra ts from A rct ium lapp a L. . [ R esult] Both ex tra ts revealed the potential antmi i robial a tiv ities againstEscherich ia col,i P roteus vulgaris, B ac illus subtilis and S tap hy lococcus aureus. A higher antmi i robial a tivi- ty was observed in 70% ethanol extra ts in omparison w ith aqueous ex tra ts, and theM IC to Ba illus subtilis and Staphy lo o us aureus was 31. 25 mg/m l, the M IC to Proteus vulgar isw as 62. 50 mg/m l, while theM IC toEscherich ia oliwas 250mg/m l; however, neither aqueous extra ts nor 70% ethanol extra ts show ed obv ious antmi