3M纸片检测大肠菌群法与国标快速检测法的比较研究.pdfVIP

安徽农业科学 ,Journal of Anhui Agri. Sci. 2009 ,37( 1) :364 - 365 ,379 　　　　　　　　　　　　　　　　　　责任编辑　王淼　责任校对　况玲玲 3M 纸片检测大肠菌群法与国标快速检测法的比较研究 1 1 2 2 2 2 范远景 , 潘丙南 , 陈 伟 , 韩明忠 , 程仁剑 , 李 艳 　 ( 1. 合肥工业大学生物与食品工程学院 ,安徽合肥 230039 ,2. 安徽凯利粮油食品公司 ,安徽合肥 231600) ( ) 摘要　[ 目的] 比较用3M 纸片法与用快速检测国标法 MEFH 对不同样品大肠菌群的检测结果。[ 方法] 以花生、小米、玉米、大米和花 生油为检测样品 , 以Alizgal 和MUGal 肉汤为培养基,设大肠菌群污染的自然状态未知浓度和模拟培养已知浓度的两种处理,进行 3M 纸 片法与MEFH 法对大肠菌群的检测结果对比试验。[ 结果] 3M 法与MEFH 中MPN 法检测自然状态的5 种样品的大肠菌群结果比较符合 率为90 % ,3M 纸片法和MEFH 中的平板法检测结果无明显差异。3M 纸片法未出现假阳性现象,证明了 3M 纸片法有很强的选择性。 [ 结论] 3M 纸片法操作简便 ,适合企业现场食品卫生检测。 关键词　大肠菌群;3M 纸片法; MPN 法;平板法 ( ) 中图分类号　TS207. 3 　　文献标识码　A 　　文章编号　0517 - 6611 2009 01 - 00364 - 02 Comparative Study on Detection Methods of Coliform Group by Using 3M Petrifilm and E . coli Count Plate with MEFHRDC ( ) FAN Yuanjing et al 　 Institute of Biological and Food Engineering , Hefei University of Technology , Hefei , Anhui 230039 Abstract 　[Objective] The study aimed to compare the results of detecting the coliform group by the method of 3M petrifilm and E. coli count plate and by microbiological examination of food hygienerapid detection of coliform bacteria (MEFHRDC) method. [Method] With peanut , millet , corn , rice and peanut oil as the tested samples and the Alizgal and MUGal broth as the medium , 2 treatments of nature with unknown concn. and simulation with known concn. for coliform bacteria group were set up to conduct the comparative test on detecting the coliform group by 3M method and MEFH RDC method. [ Result ] The results of detecting the coliform group from 5 samples in nature by 3M method was 90 % identical with the most probable nu