中草药干品和鲜品基因组总DNA提取及质量比较研究.pdfVIP

20 10 21 6 L ISH IZH EN M ED IC INE AND MATER IA MEDICA RESEARCH 2010VOL. 21 NO. 6 ( ( DNA 姚庆收, 刘秀珍, 武玉永, 蒋继来 (# , 264003) : 探讨中草药干品和鲜品总 DNA 的提取方法, 并比较干鲜品总 DNA 质量之间的差别, 为后续研究奠定基础 分别采用 SD S法和改良的 CTAB 法提取 6种中草药干品和鲜品总 DNA, 并对其进行核酸含量测定电泳检测和酶 切反应检测 改良 CTA B法提取的 DNA 质量要高于 SDS法, 在干品和鲜品样品间, 干品提取的 DNA 质量要高于鲜 品利用 CTAB 法提取鲜品和干品的各 6种 DNA 样品, 进行酶切反应得到了清晰的酶切条带 改良的 CTAB 法能 够获得高质量的中草药总 DNA, 且该方法得到的干品 DNA 比鲜品 DNA 质量更高, 酶切验证二者都可以用于进一步的实 验分析 : DNA 提取; SD S法; CTA B法; 液氮 : R284. 2; R2-03 : A : 1008-0805( 2010) 06-1570-03 Total G enom ic DNA Extraction from D ried and Fresh H erbs and Quality Comparison YAO Q ing- hou, LIU X iu-zhen, WU Y u-yong, JIANG J-i lai (D epartment of GeneticEng ineering and M olecular B iology, B inzhouM edical College, Yanta,i 264003 Ch ina ) A bstract: O b ective T o exp lore the be t total g enom ic DNA extraction m ethod from the dr ied and fre h herb , and to com pare the DNA quality a a foundation for future re earch. M e thods SD S and m od ified CTA B method w ere u ed to get DNA, and the content of nucle ic ac id, and agaro e gel electrophore i and enzyme dige tion w ere u ed. Resu lts DNA w a higher quality u ing m odified CTA B m ethod to extract, and could get h igher quality of total genom ic DNA from dry am ple . It how ed a clear enzym e dig e tion band u ing ix kind of DNA by m odified CTAB m ethod ex tracted from fre h and dried amp le of herb . Conc lu sion It can be g et high- quality of total genom ic DNA by them odified CTAB m ethod, and can be u ed for further expermi ent analy i. K ey words: DNA extraction; SD S m ethod; CTA B method; L iqu id nitrogen , , DNA 1. 3 试剂 EDTA CTA BM arker , ,