海水和淡水养殖花鲈肌肉脂肪酸组成和含量分析.pdfVIP

※分析检测 食品科学 2010, Vol. 31, No. 14 209 海水和淡水养殖花鲈肌肉脂肪酸组成和含量分析 1 1 2 1 1 许建和 ，徐加涛 ，林永健 ，罗 刚 ，毕祥静 ( 1.淮海工学院海洋学院，江苏连云港 222000 ；2 .宁波市海洋与渔业研究院，浙江 宁波 3 150 10) 摘 要：采用Folch 液回流提取投喂相同配合饲料的海水养殖花鲈和淡水养殖花鲈背部肌肉脂质，脂质经氢氧化钾- 三氟化硼法甲酯化后进行气相色谱分析。结果表明：海水养殖花鲈和淡水养殖花鲈肌肉脂肪酸组成存在一定差异。 海水花鲈肌肉脂肪酸中单不饱和脂肪酸(MUFA) 、n-3 系列多不饱和脂肪酸(PUFA) 、EPA(C20 :5 n -3)和DHA(C22 :6 n -3)含 量明显高于淡水花鲈，而饱和脂肪酸(SFA) 、n -6 系列PUFA 和AA (C2 0 :4 n -6)含量则低于淡水养殖花鲈。海水养殖花 鲈和淡水养殖花鲈肌肉中n-6 和n-3 系列PUFA 比值分别为0.27 和0.42 ，均远低于HMSO(英国卫生部)推荐的最高安 全限值(4 .0 ) 。 关键词：花鲈；脂肪酸；海水；淡水 Fatty Acid Composition of the Muscle of Lateolabrax j ap onicus (Cuvier) Grown in Seawater and Freshwater 1 1 2 1 1 XU Jian-he ，XU Jia-tao ，LIN Yong-j ian ，LUO Gang ，BI X iang-j ing (1. School of Ocean, Huaihai Institute of Technology , Lianyungang 222000, China； 2. Ningbo Academy of Ocean and Fishery, Ningbo 3 150 10, China) Abstract ：Lipids in the dorsal muscle of Japanese seabass Lateolabrax j ap onicus grown in seawater and freshwater fortified with the same feed were extracted by reflux extraction with “Folch ”solvent, esterified with KOH-BF3 method and analyzed by gas chromatography. The results indicated that an obvious difference in fatty acid profile was observed between the muscles of Japanese sea basses grown in seawater and freshwater. The muscle of Japanese seabass from seawater exhibited higher contents of monounsaturated fatty acids (MUFA), n-3 polyun saturated fatty acids (PUFA), eicosapentaenoic acids (EPA, C20:5 n-3) and docosahexaenoic acids (DHA, C22 :6 n-3), and lower levels of s