温阳化瘀中药复方对糖尿病大鼠脂肪组织中抵抗素mRNA表达影响的研究_李小娟.pdfVIP

# 2000# 辽宁中医杂志 2009年第 36卷第 11期 mRNA (辽宁中医药大学附属医院内分泌科,辽宁 沈阳 110032) :目的:研究温阳化瘀中药复方对糖尿病大鼠脂肪组织抵抗素 mRNA表达的影响, 探讨温阳化瘀中药 疗糖 尿病胰岛素抵抗的作用机制方法:采用逆转录多聚酶链式反应(RT- PCR)方法测定糖尿病大鼠腹部脂肪组织和下肢 骨骼肌间的脂肪组织抵抗素 mRNA表达结果:肥胖的 2型糖尿病胰岛素抵抗模型大鼠具有脂肪源性抵抗素 mRNA 高 表达的特点,而腹腔内脂肪组织的表达水平比周围组织骨骼肌肌间脂肪组织的表达水平更高(P 01 01)温阳化瘀中 药复方 疗可以降低脂肪源性抵抗素的 mRNA的表达水平结论:温阳化瘀中药通过降低脂肪源性抵抗素的 mRNA的 表达水平 疗胰岛素抵抗 :糖尿病大鼠; 脂肪组织;抵抗素;温阳化瘀中药复方 : R2851 5 : A : 1000- 1719(2009) 11- 2000- 03 Effects ofW arm ing-yang Dissolving-stasis Compound Chinese TraditionalM edicine(CCTM ) on Resistin mRNA E pression in Adipose Tissue ofD iabetic Rats LiX iao-juan (TheAff iliatedH osp ital of L iaoning University of Traditional ChineseM ed icine, henyang 110032, China) Abstract: Obj ective: Research the effect of arming-yang Dissolving-stasis CCTM on resistin mRNA expression in adipose tissue ofdiabetic rats, to investigate themechanism of arm ing-yangDissolving- stasisCCTM mi proving insulin resistance.M eth- ods: The reverse transcription-polymerase chain reaction(RT- PCR) wasadopted todetect the expressionsof resistin gene in ab- domen and leg intermuscularfatty tissues.Results: Fat-derivedhigh expression of resistinmRNA was character in obese type2 dia- betesmellitus rats. ResistinmRNA expressionwashigher in abdomen than leg intermuscular fatty tissues(P 0101). Treatment by arm ing-yangDissolving-stasis CCTM could decrease fat-derived resistinmRNA expression. Conclusion: arm ing-yangDisso-l ving-stasis CCTM mi prove insulin resistant by decreasing fat-derived resistin mRNA expression. Key w ords: diabetic rat; adipose tissue; resistin; arm ing-yang dissolving-stasisCCTM [ 1] Steppan