发酵木糖产乙醇菌种筛选与应用.pdfVIP

2010 No．1 · · Serial No．214 China Brewing 64 Research Report 发酵木糖产乙醇菌种筛选及应用 1 2 1，3 2 1 赵彩云 ，王异静*，关东明 ，李建飞，朱书全 （1.中国矿业大学（北京）化学与环境工程学院，北京 100083；2. 中国食品发酵工业研究院酿酒工程部，北京 100027；3.纽约州立大学） 摘 要：采用传统的菌种筛选方法，对样品进行了发酵木糖生产酒精的酵母菌的筛选，得到一株能够发酵木糖生产酒精的酵母菌，经 初步鉴定为假丝酵母菌。其在木糖含量2%、30℃、100r/min的条件下发酵，酒精产量达到3.10g/L，基本达到了一般野生酵母的水平。再 利用其发酵麦糟水解液，乙醇产量最高为4.24g/L。结果表明，Y-4T-1具有抵抗麦糟水解液中的乙酸、糠醛、羟甲基糠醛等抑制物生长 的能力，为发酵麦糟生产酒精的进一步研究提供了菌种支持，为生物质燃料生产燃料乙醇的研究提供了新的思路。 关 键 词：木糖；筛选；菌种；发酵；麦糟 中图分类号：Q93-331 文献标识码：A 文章编号：0254-5071 （2010）01-0064-04 Screening of xylose fermentation yeast to produce ethanol and its application 1 2 1,3 2 1 ZHAO Caiyun , WANG Yijing *, GUAN Dongming , LI Jianfei , ZHU Shuquan ( 1.China University of Mining and Technology, Beij ing 100083, China; 2.China National Research Institute of Food and Fermentation Industries, Beij ing 100083, China; 3.New York State University, New York, USA) Abstract: Brewer ’s spent grains is the largest number of by-product in the beer production process, a certain content of fermentable sugars can be obtained after acid hydrolysis and enzymatic treatment, including xylose and glucose. They are the main reducing sugar, which has well cost-effectiveness. In this paper, we screened the yeast which can ferment xylose to ethanol from the natural environment based on traditional strain screening methods, and they were preliminary identified as Candida yeast. The production of ethanol can be up to 3.10 g/L by fermenting xylose at 2% in 30℃,