一个新的水稻小穗梗弯曲突变体的形态特征与基因定位.pdfVIP

HEREDITAS (Beijing) 2013 2 , 35(2): 208―214 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2013.00208 一个新的水稻小穗梗弯曲突变体的形态特征 及基因定位 杨韵龙, 吴建国, 周元飞, 石春海 , 310058 60 稻穗小穗梗的发育与产量有着密切关系。文章利用 Co γ 射线辐照籼稻品种“浙农7 号”, 获得一个性 状能稳定遗传的小穗梗弯曲突变体bpb1(bent pedicel branch 1), 表现为小穗梗弯曲, 并伴有小穗梗长度增长、穗 长缩短和植株矮化等特点。扫描电镜观察显示, bpb1 突变体小穗梗的表皮毛及气孔变小, 外表皮细胞和厚壁细 胞排列不规则, 接近弯曲部位的细胞变小、排列更为紧密。bpb1 突变体小穗梗横切面观察表明, 小维管束排列 结构发生明显变化。遗传分析表明该突变表型受隐性单基因控制。利用bpb1 突变体与粳稻品种“浙农大104” 杂交构建的F2群体进行基因定位, 将bpb1 基因定位于水稻第 7 号染色体长臂SSR标记RM21537 和RM21552 之 间, 该区间的物理距离为343 kb, 该区域内未发现与水稻小穗梗发育相关的已知基因。文章为bpb1 基因的克隆 和功能研究奠定了重要基础。 水稻; 小穗梗弯曲; 突变体; 形态特征; 基因定位 Morphological characteristics and gene mapping of a novel bent pedicel branch (bpb1) mutant in rice YANG Yun-Long, WU Jian-Guo, ZHOU Yuan-Fei, SHI Chun-Hai Agronomy Department, College of Agriculture and Biotechnology , Zhejiang University, Hangzhou 310058, China Abstract: Rice pedicels are tightly associated with the yield of grain. In the present study, a novel and stable pedicel 60 mutant bpb1 (bent pedicel branch 1) was obtained from the wild type “Zhenong 7” after Co γ-ray treatment. The mutant had the typical phenotype of bent pedicel branches with multiple abnormal phenotypes, such as longer pedicels, short pani- cles, and dwarfism. Detail examination using scanning electron microscopy revealed that the pedicel epidermal hairs and stomas in the mutant were smaller than those in the wild type. The epidermal and sclerenchymatous cells were arranged irregularly, and the cells in the bend region of pedicel