响应面法优化雄烯二酮生物转化法工艺.pdfVIP

78 2010, Vol. 31, No. 24 食品科学 ※工艺技术 1,2 2 2 杨 英 ，姜绍通 ，刘 模 (1.安徽建筑工业学院环境工程系，安徽 合肥 230022 ；2.合肥工业大学生物与食品工程学院，安徽 合肥 230009) 在已有的雄烯二酮(AD)微生物转化法单因素试验最适条件基础上，采用均匀试验设计在多项影响植物甾醇 转化制备 AD 的因素中，有效筛选出主效因素：底物浓度、植物油、废糖蜜。再利用响应面分析法(RSM) 对以 上 3 个显著因子的最佳水平范围进行研究，并建立响应面及等高曲线图，比较分析了各主要因子间交互规律。通 过对二次多项回归方程求解，得到生物转化法最适条件为废糖蜜 58.49mL/L 、葵花油211.63mL/L 、甾醇6.01g/L ， 结合均匀试验最适条件：硝酸氨 3.2g/L 、磷酸二氢氨0.8g/L 、氯化汞0.055g/L 进行生物转化实验，得到 AD 产量达 到 2.55g/L ，且重复性实验结果较好。 雄烯二酮；生物转化；均匀设计；响应面分析法；优化 Optimization of Androstenedione Bioconversion by Response Surface Methodology 1,2 2 2 YANG Ying ，JIANG Shao-tong ，LIU Mo (1. Department of Environment Engineering, Anhui University of Architecture, Hefei 230022, China； 2. College of Biology and Food Engineering, Hefei University of Technology, Hefei 230009, China) Abstract ：Bioconversion processing of androstenedione (AD) is strongly affected by many factors. Uniform design has been undertaken to evaluate the effects of substrate concentration, plant oil and molasses on bioconversion rate. Through regression analysis, the amounts of sunflower oil and molasses were important factors for bioconversion processing of AD. Response surface methodology (RSM) was used to optimize the critical factors. Through the analysis of quadratic regression model equation, the optimal processing parameters for bioconversion were 211.63 mL/L sunflower oil, 58.49 mL/L molasses and 6.01 g/L phytosterol. The optimal uniform design parameters were 3.2 g/L ammonium nitrate, 0.8 g/L ammonium dihydrogen phosphate, and 0.055 g/L mercury chloride. Under the optimal conditions, th