夏涵快速提取细菌DNA方法地研究.pdfVIP

571 【检验方法研究】 快速提取细菌DNA方法的研究 夏涵，府伟灵，陈鸣，黄庆，赵猛，赵渝徽，王丰，罗阳 [摘要] 目的：研究快速、有效可以应用于压电基因传感器对病原微生物的快速检测的DNA的提取方法。方法：采 用沸水浴法，酚、氯仿法，chelexl00法和试剂盒法提取金黄色葡萄球菌ATCC25923、铜绿假单胞菌ATCC27853和大肠杆菌 ATCC 省时、成本低，适宜用于压电传感器样本的预处理。 [关键词] 细菌DNA；chelexl00法；压电基因传感器 [中圈分类号]R446．5 [文献标识码]A Medical pital，删Mait,2，yUniversity，Chongqing，400038． the forthedetee— make betweenfourmethodsofDNAextractionfrombacteriaandselectbestone Abstract：Objective：Tocomparison tionof from aUIeU8ATCC A1[℃C27853andE．coli piezoelectricgene蝴．Methods：DNAStaphylococcus25923，Pseudomormaeruginosa methodandE．Z．N．A ATCC25922W88extractedfourmethods immersion method，chelexl00 through including method，phenol／chloroform l【it．111e and ofthe weredetectedandthe shiftofthe sensordetectionw∞ gene purity output prodtlcts frepuency piezoelectric method three8tlain．8ofbacteria．Therew硇no differ- observed．Results：n伧mehtodsimmersioncouldextractDNAfromthe except significant encebetween methodandchelexl00methodintermsofthe and ofthe me phenol／chloroform purity output chelexl00methodis and and ofDNAextractedE．Z．N．Akitweie simplercheaper．nlepurityoutput by obviously themethodwas and chelexl00methodisa and methodforDNAExtra．on n眦amplicatedexpensive．CondlldOll：田le simple．rapidcheap frombacteriaandCallbe inthe of