动物益生菌对大肠杆菌抑制作用的培养条件优化.doc

动物益生菌对大肠杆菌抑制作用的培养条件优化 陈璐，苏明星刘波，黄素芳，葛慈斌 （福建省农业科学院农业生物资源研究所，福建，福州 350003） 摘 要：筛选短芽孢杆菌YSJ-0401r/min的抑菌圈为13.75-15.00 mm，pH 6.0~8.0下抑菌圈为10.50-14.50 mm。益生工程菌株YSJ-0401的最适发酵条件为培养基pH 7.5、30℃下培养48 h，摇床转速在90~210 r/min范围内均可。 关键词: 中图分类号：S816.7 文献标识码：Aermentation conditions for animal feed probiotics, Brevisbacillus brevis YSJ-0401, inhibiting against Escherichia coli Chen Lu, Su Mingxing, Liu Bo*, Huang Sufang, Ge Cibin, （Institute of Agricultural Bioresource Institute, Fujian Academy of Agricultural Sciences, Fuzhou，350003, ChinaAbstract: Screen experiment of Bacillus spp. was conducted according to the high inhibition against Escherichia coli. And then, the best fermentation condition for highest inhibition of the selected bacterium was also studied based on time, temperature, shaking speed and medium pH. The results showed that YSJ-0401, a strain of Bacillus brevis, was found to inhibit E. coli obviously. Its inhibition zones against E. coli were 10.00-13.00 mm after cultured for 24-48 h, 9.00-14.00 mm cultured at 24-39℃, 13.75-15.00 mm under the shaking speeds of 90-210 r/min and 10.50-14.50 mm at pH values of 6.0-8.0, respectively. In conclusion, the best fermentation condition for YSJ-0401 against E. coli was as follows: pH 7.5, 30℃, 48 h culture time and 90-210 r/min shaking speed. Keywords: fermentation conditions, Bacillus brevis, Escherichia coli, inhibition, probiotic 动物益生素，又称益生菌、促生素、利生剂、活菌制剂或微生态制剂等，是指摄入动物体内、参与肠内微生物平衡的活性微生物培养物。益生素能够通过直接增强动物对肠内有害微生物群落的抑制作用；或者通过增强非特异性免疫功能来预防疾病，并间接起到促进动物生长和提高饲料转化率的作用。众多实验证实了益生素具有促进增重、提高饲料转化效率、改善畜禽机体免疫功能、降低死亡率及改善环境等效果。目前使用的益生素主要是芽孢杆菌、乳酸杆菌、粪链球菌等单一菌种或复合菌种的制剂[1-]。研究用于益生素的芽孢杆菌，已报道的有纳豆芽孢杆菌[]、蜡样芽孢杆菌、枯草芽孢杆菌等[]。短芽孢杆菌YSJ-0401（Brevibacillus brevis YSJ-0401初步研究表明该菌株对畜禽肠道中有害大肠杆菌具备较强的拮抗作用可益生。为实现进一步的规模化发酵，本研究对测定了不同发酵条件下获得的益生菌发酵液对大肠杆菌K88菌株的抑制作用，了解该菌在何种条件下可获得最好的抑制能力，以确定该菌的最适发酵条件。1 材料与方法 1.试验材料 靶标菌株：禽致病性大肠杆菌（Escherichia coli）K88菌株（中国兽药监察所提供）。短芽孢杆菌YSJ-0401（Brevibacillus brevis YSJ-0401芽孢杆菌平板培养、摇瓶振荡培养和抑菌圈试验用牛肉膏－蛋白胨（NA）培养基：0.3%牛肉膏+1%葡萄糖+0.1%酵母