尖吻蝮蛇毒无出血活性纤维蛋白溶解酶的纯化和其理化性质研究.pdfVIP

J OU RN A L O F G U A N GX I M E DICA L U N IV ER SIT Y 2003 A pr ; 20 ( 2) 157 * 1 2 ( 53002 1) : ( A g kist ro do n acutu s veno m) ( No n-hemonr rhag ic f ibr ino ytic enzym e, NHF LE ) : N HF L E , , SDS-( P AG E) , : , 25 000 , , , 3 . 2 : 25 000 , ; ; ; 959 . 6; 55 ; 996 . 3 Q Q R PURIFICATION AND CHARACTERIZATION OF NON-HEMORRHAGIC FIBRINOLYTIC ENZYME FROM AGKISTRODON ACUTUS L i Zhaoyan , L ei Danqing , Y ang Bin, et a . ( Snake V enom Research Inst it ut e of Guang xi M edica Universi- ty , N anning 530021 China ) AbstractObjective: T o purif y non-hemo rrhagic fibrino yt ic enzym e( N HFL E ) f rom A gkist rodon acut u s veno m and st udy t heir physicochemica pro pert ies . Met ods: W e purify no n-hemorr hag ic f ibrino yt ic en- - , zym e by f i t ration and anion exchange chr omat og raphy and det erm ined fibrino yt ic activit y by fibrin p at e ca cu at ed mo ecu ar w eig ht by SDS-PA GE; and m easured heor rhag ic activ it y on anim a s. Result: By means of DEA E-Sepharose CL -6B , Sephadex G -75 chrom ato graphy and T SK -GEL G - 2000SW high -perf ormance ge fi t rat ion , a purif ied pro tein w ith f ibr ino y tic act ivit y w as obt ained f rom t he v enom of A gkist ro don acu - . . 25 tu s T he pro tein w as homo geneou s as judged by e ect rophr esis It s m o ecu ar w eig ht w as est imat ed t o be 000 w it ho ut any hemo rrhagic act iv ity . T he N HF L E had direct y f ibrino yt ic act iv it y and T he specif ic f ibri- no yt ic act iv ity of t he prot ein w as 3. 2 t ime higher t han t hat of cr ude v enom . Conclus