高效液相色谱法测定果蔬中7种植物激素残留量_周艳明.pdfVIP

※分析检测 食品科学 2010, Vol. 31, No. 18 301 高效液相色谱法测定果蔬中7种植物激素的 残留量 周艳明，忻 雪 (沈阳农业大学分析测试中心，辽宁沈阳 110866) 摘 要：建立高效液相色谱法测定果蔬中7 种植物激素残留的方法。选择10 种果蔬样品，经体积分数80% 甲醇提 取后、过C 18 柱净化，用带有紫外检测器的液相色谱仪测定，外标法定量。通过对该方法的精密度和加标回收实 验，所有激素的添加回收率均在70 .2 3% ～9 8.10% 之间，回收率的变异系数在3 .34 % ～10 .12% 之间，方法的最低 检出限为0.004～0.02mg/kg 、最低定量限为0.0 1～0.1mg/kg ，所建立的果蔬中植物激素残留量的测定方法满足国家标 准对食品中农药残留测定的要求，可提供给相关部门用于对相关农产品、食品的质量监控。 关键词：高效液相色谱法；果蔬；植物激素 Determination of Plant Hormone Residues in Vegetables and Fruits by High Performance Liquid Chromatography ZHOU Yan-ming ，XIN Xue (Analysis and Testing Center, Shenyang Agricultural University, Shenyang 110866, China) Abstract ：A high performance liquid chromatography (HPLC) method for determining plant hormone residues in vegetables and fruits was established. Sample preparation was accomplished after extraction with 80% methanol and clean-up on a C18 column prior to analysis using a HPLC system equipped with a UV detector and subsequent quantification by external standard method. The average spike recoveries for 10 species of vegetables or fruits such as tomato, cucumber, cap sicu m , e tc w er e b etw een 70.23% and 98.10%, with a variation coefficient ranging from 3.34% to 10.12%. The limit of detection of this method was between 0.004 mg/kg and 0.02 mg/kg, and the limit of quantification between 0.0 1 mg/kg and 0.1 mg/kg. This method meets the national requirements for pesticide determination and is applicable to food quality supervision and control. Key words ：high liquid chromatography ；fruits and vegetables ；plant hormone 中图分类号：TS207.53 文献标识码：A 文章编号：1002-6630(20 10) 18-030 1-04