D_精氨酸对菌根化马尾松植株内源多胺和抗旱能力的影响.pdfVIP

33 4 ( ) ol. 33, No. 4 2009 7 Journal ofNanjing ForestryUniversity (NaturalScience Edition) July, 2009 D * 徐 超, 吴小芹 , 张红岩 (南京林业大学, 江苏省有害生物入侵预防与控制重点实 室, 江苏 南京 210037) :为研究内源多胺对菌根化马尾松植株抗旱能力的影响, 以分别接种 4种外生菌根真菌及其混合菌的 5种 菌根化马尾松小苗为材料, 通过添加 ADC竞争性抑制剂 D精氨酸(DArg) 进行室内模拟自然干旱胁迫处理,分 析了各处理植株的萎蔫度针叶中腐胺 ( Put)亚精胺( Spd) 和精胺 ( Spm)含量的变化及其与抗旱能力的关系 结果显示:未施加 DArg的处理中, 各植株的抗旱能力依次为接种混合菌根菌的植株较高紫金蜡蘑( La)菌根化 植株次之CK植株较低, 而 DArg处理的各植株抗旱能力发生了逆转, 依次为 CK植株较高La菌根化植株次 之接种混合菌根菌的植株较低分析表明菌根化马尾松植株 Put合成主要依赖以 LA rg为前体的途径, 并且向 Spd和 Spm 的转化效率较 CK植株高; 维持较高 Spm 含量( Spd+ Spm) /Put值, 是菌根化马尾松植株抗旱的重要 原因之 一 : D精氨酸; 外生菌根; 内源多胺; 马尾松;抗旱能力 : S718; Q945 : A : 1000- 2006( 2009) 04- 0019- 05 Impact ofDArg on drought resistance and endogenous polyam ines inmycorrhizalP inus m asson iana * XU Chao, WU X iaoqin , ZHANG Hongyan ( Jiangsu Key Laboratory forPrevention andM anagement of Invasive Species, NanjingForestry University, Nanjing 210037, China) Abstract: W e studied the drought resistance ofmycorrhizalP inus m asson iana caused by endogenous polyam ines follow ing a drought stress in the greenhousew ith the treatment of DArg. The results showed that, in drought stress without treatment ofDA rg, the strength of drought resistance is in the order ofP. m asson iana inoculated bym ixed fungi, inocu lated by La and control plants. On the contrary, P. masson iana dealtwithDA rg and drought stress had taken place re versely. MycorrhizalP. masson iana canmaintain high content of Spm and high ( Spd+ Spm) /Put value, in which the biosynthetic of Put reliesmainly on LArg precursor, and its conversion to Spm and Spd ismore e