Lecture40amp;41Edible Vaccines.ppt

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Lecture40amp;amp;41EdibleVaccines.ppt

Downloaded from /knowledge Lecture4041 Edible Vaccines * * EDIBLE VACCINES FROM PLANTS Two strategies for production 1) Expression of foreign antigens in plant via stable transformation 2) Delivery of vaccine epitopes via plant virus (Mason and Arntzen, 1995) Strategies for the production of candidate vaccine antigens in plant tissues (Mason and Arntzen, 1995) HEPATITIS B SURFACE ANTIGEN IN TOBACCO Gene encoding HBsAg expressed in tobacco plants Targeted to the chloroplast Genetic constructs- 1) driven by CaMV 35S promoter 2) 5 UTR from TEV and TMV 3) signal peptide from soybean VSP 4) A plastid transit peptide Contd... Chloroplast of tobacco Contd... Structures of expression cassettes Contd... Various expression vectors were constructed pHB110 was plastid targeted Expression level 0.01% of soluble protein (Richter et al., 2000) Contd...

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