海参花水提物对环磷酰胺诱导小鼠生殖系统受损保护作用.pdfVIP

海参花水提物对环磷酰胺诱导小鼠生殖系统受损的保护作用  刘坤，高华，张婕，石振艳，刘占涛，郭锡春(青岛大学药学院，山东 青岛 266021) 摘要：目的 探讨海参花水提物对环磷酰胺诱导的小鼠生殖系统受损、卵巢早衰的保护作用。方法 昆明♀小鼠随机分 为空白组、模型组、阳性对照组(戊酸雌二醇0.085 mg·kg1) 、海参花水提物低、中、高剂量组(分别为 150，300 ，600 mg·kg1) ， 除空白组每天注射生理盐水外，其余各组连续5 d 腹腔注射环磷酰胺28 mg·kg1 ，造成小鼠生殖系统紊乱，建立小鼠卵巢 早衰模型，并同时灌胃给予相应药物。放免法检测血清雌二醇含量(E2) 、孕酮(P)含量，比较各组小鼠间子宫、卵巢、脾、 胸腺等脏器重量，并进行小鼠卵巢、子宫组织形态学观察。结果 连续用药 4 周后，与模型组相比，海参花水提物各剂 量组均能显著增加小鼠的子宫、卵巢、胸腺等脏器重量(P0.05 或P0.01) ，显著提高E2 、P 含量(P0.05 或P0.01) ，增 加子宫内膜平均厚度(P0.01) ，改善卵巢形态。结论 海参花水提物可增加子宫内膜厚度，对卵巢早衰发挥防治作用，对 磁性小鼠生殖系统有一定的保护作用。 关键词:海参花；环磷酰胺；卵巢早衰；生殖系统 中图分类号：R965 文献标志码：A 文章编号：1007-7693(2014)07-0802-04 DOI: 10.13748/ki.issn1007-7693.2014.07.007 Protective Effect of Water Extracts of Sea Cucumber Ovum on Reproductive System of Premature Ovarian Failure Mice LIU Kun, GAO Hua, ZHANG Jie, SHI Zhenyan, LIU Zhantao, GUO Xichun(School of Pharmaceutical Science, Qingdao University, Qingdao 266021, China) ABSTRACT:OBJECTIVE To investigate the protective effects of water extracts of sea cucumber ovum on deficiency of the genital system injured by cyclophosphamide. METHODS Female Kunming mice were selected and randomly divided into 6 groups included control group, model group, positive group (estradiol valerate 0.85 mg·kg1), and low-, middle-, high-dose sea cucumber ovum group(150, 300, 600 mg·kg1) with 10 animals in each group, except the control group injected with normal saline, others were injected with cyclophosphamide(28 mg·kg1) daily for successive 5 d to induce reproductive system disorders and premature ovarian failure in mice. Detected the level of estradiol(E2) and progesterone(P) by radioimmunoassay, all mice were compared the weight of organs, such as uterus, ovarian, spleen, and thymus, observed for the form of uteru